FastQCFastQC Report
Mon 4 Sep 2023
EGAF00007987539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007987539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46762574
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTACAGGTAAGTAGTCCCTGATATA4173840.8925599347888762No Hit
TTAGGCAGTCTCTCTGGAACAACGCC3865640.8266525277244148No Hit
ATGGGAGAGGAACTGCCGTACAATAG3789190.8103039836943108No Hit
ATGGGAGAGGAACTGCTCTAGGTCGA3678520.7866376217870299No Hit
GGCTCGATCAGTGCATTCAGACGGAT3577000.7649279528539211No Hit
ATGGGAGAGGAACTGCATCAACATGC3496640.7477432700774769No Hit
TTAGGCAGTCTCTCTGAAATCATATA3404990.7281442634017538No Hit
CAGATCAGTAGAAGGATAGCGCTTAG3403430.7278106632881244No Hit
AACTTTCTCGGCGCTATTTTACGGTT3370390.7207451839584365No Hit
TATGCCCTCCAACCAACTCACTAACC3329860.7120779963908744No Hit
AAACCTGCACAGGAGTTTAGCACGGT3238850.69261585130023No Hit
AGATCTGGTGTTTGGTATGCAAACTG3139710.6714151363866325No Hit
AAGTCTGCAGCGTTCGACATCCTTGT3082550.6591916860692912No Hit
AGAATAGTCAGCTGGCTATCGCAGGC3041720.6504603446337236No Hit
AAGGCAGGTACGCACCGCTTAGGCGC3032300.6484459131783464No Hit
AACTCAGGTGATGTGGGCGACCTTCG2971910.6355317395488109No Hit
GTGGGTCAGATCCCGCGATACGTTCC2953690.6316354612986017No Hit
ACTATCTGTAAGGATTCGGGTGCTAC2948890.6306089994105115No Hit
AAACCTGCACAGGAGTAAACTCATCC2824000.6039017441597633No Hit
TGCTGCTAGATGAGAGAAAGCCTACG2796690.5980616037089831No Hit
GTGGGTCAGATCCCGCGCATTTGTGT2786320.5958440183382548No Hit
TGCACCTCAATAAGCAGCCATTCACG2786250.5958290491023869No Hit
CACATTTAGCGTGAGTATAAGGCTCA2777170.5938873253640828No Hit
AAACCTGCACAGGAGTAGGATAGCGC2750580.5882011541965162No Hit
ACTATCTGTAAGGATTGACATTGCGC2745780.587174692308426No Hit
AGAATAGTCAGCTGGCAATGAGGCTA2738830.585688461032962No Hit
CACATTTAGCGTGAGTTCACTGCCCT2734680.5848009991922173No Hit
AAACCTGCACAGGAGTCCGGCATGGC2728320.5834409371904977No Hit
AAGTCTGCAGCGTTCGGTCGTTGCGT2726280.5830046908880593No Hit
TGCACCTCAATAAGCACTTGACTTGA2701980.5778082275796025No Hit
TTTGGTTGTGTCGCTGACGACGTGTC2699470.5772714735506219No Hit
CTTGGCTGTATGCTTGCCCAACACTA2694270.5761594731718575No Hit
CAGATCAGTAGAAGGATGGTTAACAC2681970.5735291645836262No Hit
GAACCTACAATCCGATAGTTGACTGA2678440.5727742874034265No Hit
AAACCTGCACAGGAGTAAGCACAATC2677360.5725433334786062No Hit
GTACTCCAGCCTATGTTCCTATCAGC2642290.5650437463087468No Hit
TGCTGCTAGATGAGAGTTCGATTAGG2638830.5643038383644151No Hit
CAGCATATCCAGAGGATCGGAGAATA2631560.5627491762964117No Hit
GAGCAGACATGCAATCCAAACACCCT2617530.5597489137360147No Hit
CTCTGGTTCTCCGGTTAACATCAGCA2616980.5596312983113376No Hit
GTGGGTCAGATCCCGCATTGCCATCT2595120.5549566197959933No Hit
ACGGGTCCATTTGCTTTATCACTCGG2593230.5545524504275577No Hit
GTGGGTCAGATCCCGCTGGTTCCACA2590330.5539322963701698No Hit
CACAGTAGTATAGTAGTCCCCATCAA2577480.5511843723572616No Hit
TGCTACCCATGGTAGGCTATTTTTGG2572530.5501258335351685No Hit
CACATTTAGCGTGAGTCGAATATGTC2562770.5480386943627183No Hit
TGCACCTCAATAAGCATTCGACTCAT2542920.5437938467630119No Hit
GTGGGTCAGATCCCGCACTGGTTGAA2537180.5425663694218372No Hit
TGCTACCCATGGTAGGTATAGACTCT2526570.5402974609567044No Hit
GTACTCCAGCCTATGTTGAGTTTTCC2520940.5390935067004652No Hit
CTTGGCTGTATGCTTGGAATGCACGG2516380.5381183679067795No Hit
ACGGGTCCATTTGCTTCGAATAAGGG2493220.533165689296744No Hit
AAACCTGCACAGGAGTTGATACCTCC2470800.5283712568944559No Hit
AAACCTGCACAGGAGTTTTTAGTCGA2457400.5255057174568706No Hit
TGCTGCTAGATGAGAGCAGCCCACAA2442440.5223065779056559No Hit
AGAATAGTCAGCTGGCAGGCTTGTGT2427180.5190432844864357No Hit
TGCTGCTAGATGAGAGGACACGCATG2418970.517287606965348No Hit
GTGGGTCAGATCCCGCCGTTGTACAC2414130.5162525912281903No Hit
CTTGGCTGTATGCTTGTTAAGACGGA2412360.5158740834069571No Hit
AAACCTGCACAGGAGTATGATGGGCA2404490.5141911136029423No Hit
AGGCCACTCTGCGTAAGGTTGGCTCA2402790.513827575017577No Hit
AAGTCTGCAGCGTTCGCTAACCCCAA2402730.5138147442439759No Hit
CGGAGCTCACGACTCGTGGCGCCACG2372170.5072796035564681No Hit
ATGGGAGAGGAACTGCAGTAAGGAGC2355570.5037297561934893No Hit
GTGGGTCAGATCCCGCGGACCCTGGA2340750.5005605551140107No Hit
GGCTCGATCAGTGCATCATAAAACAG2335730.4994870470560495No Hit
GGACAGAAGCAGCCTCTTAACCCCAG2326900.4975987848744169No Hit
ATCATCTCAATCTGCACCCGTCGACA2310900.49417724524744944No Hit
TGCCCATAGCCCAGCTCTATCGCCGT2297300.49126893656452697No Hit
AGAATAGTCAGCTGGCTTTGCGGCCT2293870.49053544400699584No Hit
AGAATAGTCAGCTGGCGAGGACGCAG2288460.48937853592062747No Hit
AGAATAGTCAGCTGGCGGCGGGTCTC2285910.4888332280425795No Hit
CACATTTAGCGTGAGTTTAATCTGAA2277500.4870347812761547No Hit
GGCTCGATCAGTGCATGTGCGGAGCC2268780.4851700421794575No Hit
GTGGGTCAGATCCCGCACCTACATAA2260450.4833887031111675No Hit
AGAATAGTCAGCTGGCGTGTCTATCA2259680.48322404151661974No Hit
GCTCCTATCCGTACAAATCAGAACAA2249440.48103425615536044No Hit
AAGTCTGCAGCGTTCGGTCTCTGCTC2233410.4776063011415924No Hit
AAGTCTGGTAGCCTCGACCCGTTTTC2232310.4773710702922384No Hit
TTTGGTTGTGTCGCTGACCCTATCGT2221680.4750978849025719No Hit
ACGGGTCCATTTGCTTGCGTCCGTGT2217860.4742809923166334No Hit
GAAACTCCAGTATGCTAAACGTCTGC2199630.4703825756041573No Hit
AAGGCAGGTACGCACCTAGACTACGC2194670.4693218983197973No Hit
TGCCCATAGCCCAGCTAATCCAGCGT2193070.4689797443571006No Hit
GAAACTCCAGTATGCTTCGGTTAACG2184670.4671834360529427No Hit
AAGTCTGCAGCGTTCGTAGACGTGGG2183350.4669011590337178No Hit
GGCTCGATCAGTGCATGCTTCAATGT2182580.46673649743917006No Hit
TGCTGCTAGATGAGAGAATCCTCCGA2180750.46634515884433564No Hit
TCGTACCAGCTGCAAGCCCGCAGAGC2173350.4647626967668632No Hit
ACGGGTCCATTTGCTTCAGACTTCCG2156710.46120429555481696No Hit
CAGATCAGTAGAAGGAGTCTCAATCC2142960.4582639099378918No Hit
CAGATCAGTAGAAGGAGACTTGCAGC2139920.45761381740876794No Hit
AGAGCGATCAAGAAGTCGATAGGGCT2128100.45508615500934574No Hit
ATGGGAGAGGAACTGCCGACTATACC2124250.45426284703660663No Hit
ACGGGTCCATTTGCTTTACACAGTAA2123120.45402120080045205No Hit
ATCATCTCAATCTGCATTCGGGTCCC2108030.4507942612397684No Hit
TGCTACCCATGGTAGGTCTCGTGCCT2107290.4506360150320211No Hit
ATGGGAGAGGAACTGCGCGTACAGCT2097630.44857026048223947No Hit
AAACCTGCACAGGAGTTAAAACTGCG2093210.4476250601602897No Hit
CGGAGCTCACGACTCGTACTGTCTAT2087510.4464061366681826No Hit
TGCTACCCATGGTAGGTGATCTTGAG2071460.44297390472988074No Hit
GTGGGTCAGATCCCGCCCAATACTCA2068520.4423451968234255No Hit
CGGTTAACATGCATTACAAGTGTTCT2065940.441793473558577No Hit
AACTCAGGTGATGTGGTACCCGTCAG2055920.4396507343671886No Hit
CAGATCAGTAGAAGGACCGCCCTTAT2043180.4369263334392157No Hit
ATCCGAAAGACGCACACGGCAGCGTG2038700.4359683023436648No Hit
CGGAGCTCACGACTCGTAGATTGCTC2035460.4352754405692039No Hit
AAGTCTGCAGCGTTCGTCTGGGTCTT2030200.43415060941683836No Hit
AAACCTGCACAGGAGTAGGTCTTCCC2021760.432345747263613No Hit
CAGCATATCCAGAGGAGCTAACCTAC2019800.4319266086593095No Hit
GCCTCTAGTCGCGGTTCGCCCTGGCT2016580.43123802380938225No Hit
CACAGTAGTATAGTAGCTAGGAAGGT1972570.4218266513729548No Hit
CGCGGTACAAAGGAAGAGTCGTTCGC1967470.4207360356168589No Hit
TCGTACCAGCTGCAAGATTGCCCTGA1964810.4201672046538756No Hit
AGTCTTTCAAACTGTCCTCAACCCTC1954390.417938926971813No Hit
CACATTTAGCGTGAGTTCCTTGTCTT1945830.4161084032713854No Hit
TGGTTCCGTCCAAGTTGTCAGCGTTG1940520.41497287980768555No Hit
AACTCAGGTGATGTGGACAACGGGAT1924810.4116133555864568No Hit
GGTGAAGCAATGGAGCTATCTTAAGT1897900.4058587536263508No Hit
ATCCGAAAGACGCACAGCCTGAGTGG1887510.4036368913310888No Hit
CCCATACTCCAAAGTCATGTAGAGTG1879720.40197102922520905No Hit
TGTTCCGCAATAAGCAGGTTTCCCAA1878960.4018085060929281No Hit
GGCTCGATCAGTGCATAAATGTTTGC1863010.3983976587772948No Hit
GTGGGTCAGATCCCGCGCCCTATCTG1855640.3968216120866229No Hit
ATGGGAGAGGAACTGCCTCTCACATC1831180.39159093338189643No Hit
AGAATAGTCAGCTGGCTGTACAACGC1831120.3915781026082953No Hit
CTTGGCTGTATGCTTGTATGCGCGGG1826140.39051314839940166No Hit
GGCTCGATCAGTGCATCCTGTAGTCT1816240.3883960707552155No Hit
AACTTTCTCGGCGCTACTTGTGGCAA1814530.38803039370758335No Hit
AAGGCAGGTACGCACCAAAATCCGAG1795310.3839202692306886No Hit
ATAACGCGTTCCACGGTCCATTAGTC1794590.3837662999474751No Hit
TGGTTCCGTCCAAGTTGTATTGACGG1779350.38050728345278856No Hit
ACGAGGACACACTGCGAAGAGGCCTT1778020.38022286797129684No Hit
ACTATCTGTAAGGATTTTCAGCCCTG1752100.3746799737756095No Hit
GCTTCCAGTGCGAAACCGCGTATCAA1742760.37268265001836726No Hit
GTACTCCAGCCTATGTAATTATGAAC1739740.3720368344137771No Hit
CGGAGCTCACGACTCGGCCATTCCCC1737820.3716262496585411No Hit
AAACCTGCACAGGAGTCAACTCTCCG1716610.3670905711905423No Hit
CTCGAGGGTAACGCGATGGGATGAGG1714620.36666501719943817No Hit
CACAGTAGTATAGTAGTCTAGTCAAA1701680.3638978470261282No Hit
GGCTCGATCAGTGCATCTCGTAGCGG1696830.3628606928267037No Hit
GTGGGTCAGATCCCGCAAAGGCGGTA1691420.36170378474033527No Hit
AGAATAGTCAGCTGGCCAGAGGCTCG1666810.3564410291016059No Hit
CGGTTAACATGCATGTGGGCGGATGC1638760.35044264244307854No Hit
ACTATCTGTAAGGATTCATATGCATA1637190.35010690386718235No Hit
TTGACTTGTCATTAGCAAGGGTTATT1637140.3500962115558481No Hit
CACATTTAGCGTGAGTGTAAGACGTG1635720.3497925499139547No Hit
AGAATAGTCAGCTGGCAATGCTTCAT1606720.3435910093400761No Hit
TTTATGCCAGCTGCACTGAAAATGCG1599000.3419401164700643No Hit
AGAATAGTCAGCTGGCCCTGAGTCCA1598720.34188023952659236No Hit
AAACCTGCACAGGAGTCGCGGTCTAT1563360.3343186369509942No Hit
TTTATGCCAGCTGCACCGCGCGCTCT1556050.3327554210339234No Hit
TTTGGTTGTGTCGCTGTGGGTTCTAC1514930.3239620641926169No Hit
AAGTCTGCAGCGTTCGAGCATACGCG1513340.3236220486921871No Hit
ATCTACTTCCCACTTGACGCCCCTAG1500630.3209040631510147No Hit
GTAGGCCCAGCAGTTTGATCATATGG1488130.31823098531744637No Hit
TCGTACCAGCTGCAAGATTAGTAAGT1433800.30661271982162486No Hit
CTAAGACGTTAAGTAGGGCCACAAAG1364510.29179531477458875No Hit
TGGTTCCGTCCAAGTTCTTGTGGTCA1263900.2702802459077638No Hit
ATCCGAAAGACGCACACCCATCATCG1261760.26982261498265686No Hit
ATCTACTTCCCACTTGAGGGATCAGC1233820.26384775140906486No Hit
AGAATAGTCAGCTGGCGATAAATGCG1224750.26190816613302764No Hit
AAGTCTGCAGCGTTCGGAGAGGGTAA1224550.26186539688769056No Hit
GTACTCCAGCCTATGTACTATTTAGG1188060.25406214807593785No Hit
CCTTCCCCAGGACCCTACTAGACCCA1186370.2537007479528394No Hit
CCTTCCCCAGGACCCTGATTGCGACT1167610.24968899274021997No Hit
ACTATCTGTAAGGATTAACGCAAACT1158570.24775582285098333No Hit
CCTTCCCCAGGACCCTGGCAACGCGC1132280.2421338055514224No Hit
TAGTGGTGTGCTGTATACAAGTCATA1097340.23466201839103212No Hit
ACTATCTGTAAGGATTAAGCACGTAG1089310.2329448331907478No Hit
CTCGAGGGTAACGCGAAGCCGTGGGT1084010.23181144818931482No Hit
GTACTCCAGCCTATGTGACTCGTCGA1073560.22957675512045167No Hit
TCGTACCAGCTGCAAGATAGTTCCCG1065490.22785101607109992No Hit
GTGGGTCAGATCCCGCGTCTGTTACT1060130.22670480029606585No Hit
CCGTGGACAGATCGGAGGGCAATACC1035600.22145915235547128No Hit
ACGGGTCCATTTGCTTTGCCTATACT981890.20997347152019477No Hit
CTTGGCTGTATGCTTGCACTGTTCAG971560.20776443999853386No Hit
GGTGAAGTCTTAGCATGTACTGCTCG941260.20128489932996416No Hit
AGGCCACTCTGCGTAACGGATAACTT938260.20064336064990776No Hit
AGAATAGTCAGCTGGCGCACCATGCG872430.18656586354720336No Hit
GAAACTCCAGTATGCTAGGTCACTTA839460.17951535345338346No Hit
TGGTTCCGTCCAAGTTAACTCTGAGT834260.17840335307461902No Hit
CCTTCCCCAGGACCCTCAATTGCCTT805310.1722125048120747No Hit
GTACTCCAGCCTATGTATCAATCGTT793700.16972975012025643No Hit
ACGTCAATCGCAAACTCGACAACACG737530.15771800756733367No Hit
TGCTACCCATGGTAGGCTTCATCCAC699110.14950203553807795No Hit
AGAATAGTCAGCTGGCTTTTCAAGTT657910.14069157099863666No Hit
ACACCGGGTAGGAGTCCCCGTTGACT651090.13923313973264176No Hit
ATAACGCGTTCCACGGGACGCTCGCT528850.11309257698261008No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)