FastQCFastQC Report
Mon 4 Sep 2023
EGAF00007987633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007987633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34756320
Sequences flagged as poor quality0
Sequence length91
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC19410025.584601591883145No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC9169172.6381302738609844No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC6889571.9822495592168559No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGACTG4236911.2190329701188158No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC3263580.9389889378392189No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2082840.5992694278335566Clontech SMART CDS Primer II A (100% over 26bp)
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC1611440.46363941867263275No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT1262040.36311093924788357No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATG1209630.3480316673341712No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT838640.24129136801594644No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG838200.24116477233493075No Hit
GTTTGATGTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTGCCCACAG826040.23766612805958742No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGACTGA676610.19467250848191062No Hit
GGTTGGGGTTTGATGTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTG635340.1827984090375506No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC586910.16886425260211668No Hit
GTCTGAGATCTGACCCCAAAGAAAGTGCCCAGGAGAAGGGCAAGGAGAGA544390.15663050633668926No Hit
GCTGGCCATTGATGAATAGTCTGAGATCTGACCCCAAAGAAAGTGCCCAG524410.15088191154874855No Hit
GGCTGCACCAGGGTCGCTGGCCATTGATGAATAGTCTGAGATCTGACCCC515540.14832985770645454No Hit
GTTGGGGTTTGATGTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTGC509430.14657190404507725No Hit
GGCCAATACCAACGGAGTAGAAGAGCAGCTGGAGGCCCCTGCCTGCAGCC484710.13945952851164908No Hit
GTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTGCCCACAGGCTGCAC451490.1299015545949629No Hit
GCCCACAGGCTGCACCAGGGTCGCTGGCCATTGATGAATAGTCTGAGATC448520.12904703374810683No Hit
GATGAATAGTCTGAGATCTGACCCCAAAGAAAGTGCCCAGGAGAAGGGCA447050.1286240890865316No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT444170.1277954628107924No Hit
GGTTTGATGTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTGCCCACA438790.12624754289291845No Hit
GGTACCAGTATAGGTTGGGGTTTGATGTTCCCTCCACAGTGCACTCCAGA437080.12575554604169834No Hit
GGCCCCTGCCTGCAGCCTGTCGGTACCAGTATAGGTTGGGGTTTGATGTT435720.12536425030037704No Hit
GCCTGTCGGTACCAGTATAGGTTGGGGTTTGATGTTCCCTCCACAGTGCA422180.12146855593457535No Hit
GCAGCTGGAGGCCCCTGCCTGCAGCCTGTCGGTACCAGTATAGGTTGGGG412610.11871509987248362No Hit
GCTGGAGGCCCCTGCCTGCAGCCTGTCGGTACCAGTATAGGTTGGGGTTT410600.11813678778420732No Hit
GGATGAACTGCCGGTCCTGGGGTCTGGAGGCTGAGAGATTCTGGGGCACC379610.10922042379630525No Hit
GATGTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTGCCCACAGGCTG378310.10884639110239518No Hit
GGAGGCTGAGAGATTCTGGGGCACCTCAGAGCTGATCTGGCCAATACCAA372870.10728120813711002No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG359710.10349484640491283No Hit
GGCTGCCCACAGGCTGCACCAGGGTCGCTGGCCATTGATGAATAGTCTGA354640.1020361188986636No Hit
GGGTTTGATGTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTGCCCAC348680.10032132285581442No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)