FastQCFastQC Report
Mon 4 Sep 2023
EGAF00007987636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007987636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34577562
Sequences flagged as poor quality0
Sequence length91
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC19275585.574591985403714No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC9083572.627012858801323No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC6875911.9885467922810751No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGACTG4209041.217274948418862No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC3241780.9375386269280639No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2046790.5919416759342374Clontech SMART CDS Primer II A (100% over 26bp)
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC1604570.46404948966615983No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT1262130.36501416727992564No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATG1193200.34507927424148643No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG844260.24416412007301153No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT831710.24053459870883898No Hit
GTTTGATGTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTGCCCACAG811620.23472447247726722No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGACTGA673700.19483733410701426No Hit
GGTTGGGGTTTGATGTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTG629340.18200820520544508No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC582810.16855150169349706No Hit
GTCTGAGATCTGACCCCAAAGAAAGTGCCCAGGAGAAGGGCAAGGAGAGA540910.15643381682028362No Hit
GCTGGCCATTGATGAATAGTCTGAGATCTGACCCCAAAGAAAGTGCCCAG516920.14949579151936737No Hit
GGCTGCACCAGGGTCGCTGGCCATTGATGAATAGTCTGAGATCTGACCCC514990.14893762608248667No Hit
GTTGGGGTTTGATGTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTGC500280.14468342215683108No Hit
GGCCAATACCAACGGAGTAGAAGAGCAGCTGGAGGCCCCTGCCTGCAGCC478220.13830356229279556No Hit
GATGAATAGTCTGAGATCTGACCCCAAAGAAAGTGCCCAGGAGAAGGGCA449750.13006989908658106No Hit
GCCCACAGGCTGCACCAGGGTCGCTGGCCATTGATGAATAGTCTGAGATC448290.1296476599478008No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT445480.12883499420809366No Hit
GTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTGCCCACAGGCTGCAC444210.1284677039983328No Hit
GGTTTGATGTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTGCCCACA439110.12699275906149773No Hit
GGTACCAGTATAGGTTGGGGTTTGATGTTCCCTCCACAGTGCACTCCAGA435310.12589378048111086No Hit
GGCCCCTGCCTGCAGCCTGTCGGTACCAGTATAGGTTGGGGTTTGATGTT434130.12555251871141176No Hit
GCCTGTCGGTACCAGTATAGGTTGGGGTTTGATGTTCCCTCCACAGTGCA416360.12041334782365513No Hit
GCTGGAGGCCCCTGCCTGCAGCCTGTCGGTACCAGTATAGGTTGGGGTTT412550.11931147719437248No Hit
GCAGCTGGAGGCCCCTGCCTGCAGCCTGTCGGTACCAGTATAGGTTGGGG407760.11792618577330582No Hit
GATGTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTGCCCACAGGCTG378640.10950453938886728No Hit
GGATGAACTGCCGGTCCTGGGGTCTGGAGGCTGAGAGATTCTGGGGCACC375280.10853281095989359No Hit
GGAGGCTGAGAGATTCTGGGGCACCTCAGAGCTGATCTGGCCAATACCAA366390.1059617794915674No Hit
GGGTTTGATGTTCCCTCCACAGTGCACTCCAGAGAGAGCGGGCTGCCCAC350880.10147621165425139No Hit
CAGGCAGTATCTGGAGTCATTGAGGGCGGGCTGCTCCTTGAGGGGCTGCG350620.10140101838296177No Hit
GGCTGCCCACAGGCTGCACCAGGGTCGCTGGCCATTGATGAATAGTCTGA346770.10028757955809608No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)