FastQCFastQC Report
Mon 4 Sep 2023
EGAF00007987637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007987637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30728345
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAGAGT973714231.687817876296297No Hit
AGTCCCAC762291424.80743430861636No Hit
CTATAGTG669277921.78047337075915Clontech Universal Primer Mix Short (100% over 8bp)
TACGTTCA636338320.70851196183849No Hit
AGTCCCAA350460.11405104960908242No Hit
CTATAGTA334230.10876928126132403No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)