FastQCFastQC Report
Tue 23 May 2023
EGAF00008003181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50738477
Sequences flagged as poor quality0
Sequence length102
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTACGAGCAGTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14275252.8134959588952584No Hit
AGCTACGAGCAGTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8201971.6165187614914023No Hit
AGCTACGAGCAGTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6728891.326190772340289No Hit
CCTATTCCGATTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6618511.3044360791515284No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5138641.012769855114098No Hit
CCTATTCCGATTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5084311.0020620051327123No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4950670.9757230198297044No Hit
CCTATTCCGATTCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4813610.9487099898564161No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4202460.8282589956336293No Hit
ACCCGTCCCTAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3827690.7543959192941483No Hit
CCTATTCCGATTCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3588450.7072443266280933No Hit
TCGCTTGGATGGTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3380340.6662281171742699No Hit
ACCCGTCCCTAAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3269100.6443039273725145No Hit
TCGCTTGGATGGTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2715780.5352505949281844No Hit
TCGCTTGGATGGTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2609160.5142369566985623No Hit
CCTATTCCGATTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2382610.46958642451960075No Hit
GCACTCCTGCATGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2345170.4622074091817931No Hit
ACCCGTCCCTAAGAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2036680.4014073973879823No Hit
TAACTCAGGGCCTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2035560.40118665761291966No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1942150.38277656619452727No Hit
GCACTCCTGCATGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1914470.3773211403251225No Hit
TTCCGAGGATGATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1913920.37721274132843996No Hit
TAACTCAGGGCCTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1865970.3677623197085715No Hit
TGGATTCCCGGACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1754250.345743527146075No Hit
TGGATTCCCGGACTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1576180.3106478738019669No Hit
TTCCGAGGATGATCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1558440.30715151343624286No Hit
GGGTTGTTGTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1499490.29553311188272363No Hit
CATTAGAGTCTGCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1476730.2910473643109154No Hit
TAACTCAGGGCCTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1472440.29020185213679156No Hit
GCACTCCTGCATGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1451600.2860945156079478No Hit
AGCTACGAGCAGTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1415890.27905646438697795No Hit
CATTAGAGTCTGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1359520.2679465526724423No Hit
GGGTTGTTGTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1315180.25920762264898095No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1286540.25356299125809395No Hit
GGGTTGTTGTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1260530.2484367041604343No Hit
TCAGGTCCTTTCAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1209120.2383043543068902No Hit
TCAGGTCCTTTCAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1206680.2378234569397895No Hit
AGCTACGAGCAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1149110.226477038323401No Hit
CTTCCGATTCATTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1086950.21422598080742553No Hit
GCATTCTGTCACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1056020.20813001541216936No Hit
TGGATTCCCGGACTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1033480.20368762743903407No Hit
AGCTACGAGCAGTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT997150.19652738098544031No Hit
CAATCAGACCTATGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA987630.194651092897408No Hit
CATTAGAGTCTGCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA965970.19038214331896483No Hit
CATTAGAGTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960560.18931589136977842No Hit
CTTCCGATTCATTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA929930.18327905269998546No Hit
TCAGGTCCTTTCAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA905030.1783715344865397No Hit
TTCCGAGGATGATCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA870830.17163108778373462No Hit
CAATCAGACCTATGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA847030.1669403675636539No Hit
ACCCGTCCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA814880.1606039534848474No Hit
AGCTACGAGCAGTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC812900.16021371709679028No Hit
CTTCACTCTGTCAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770560.15186896524308366No Hit
AGCTACGAGCAGTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT755460.1488929200614358No Hit
CAATCAGACCTATGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA753910.14858743197987595No Hit
TCAATCCTTCCGCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA723360.14256636043687318No Hit
AGCTACGAGCAGTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT711300.14018946607325244No Hit
CTTCACTCTGTCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA706050.1391547483776464No Hit
CCTATTCCGATTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT695720.1371188181308635No Hit
GCAGAAATCTCCCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA678370.13369932250824162No Hit
GCAGAAATCTCCCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA675510.13313564772549244No Hit
CCCGCAGTATAACGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA672430.13252861334407023No Hit
ACCCGTCCCTAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA668720.1317974128391753No Hit
TGAAGGCTCATTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA661920.13045720706200936No Hit
CCTATTCCGATTCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650130.12813352675130554No Hit
AGATTCCTTCGTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA624580.12309790063268947No Hit
AGCTACGAGCAGTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC606350.11950496661537555No Hit
AGCTACGAGCAGTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG606180.11947146147094641No Hit
AGCTACGAGCAGTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT581310.11456985593004693No Hit
CTTCCGATTCATTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA576140.11355090536123108No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT574280.11318431966335922No Hit
AGATTCCTTCGTGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA555920.10956576406501124No Hit
TTCCGAGGATGATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA554790.10934305339909986No Hit
CCTATTCCGATTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT550640.10852513369685889No Hit
GCAGAAATCTCCCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA538960.10622313318549156No Hit
GCATTGTACGATTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA536780.10579347898045895No Hit
CATTAACGGGATGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA534220.10528893092317296No Hit
TGGTAACGACAGTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA523540.10318401949668296No Hit
GCACCAACAGCCATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520870.10265779164006046No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT514090.10132152764459208No Hit
CCCGCAGTATAACGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA513210.10114808924990004No Hit
TTCCGAGGATGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA511160.10074405662590148No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)