FastQCFastQC Report
Tue 23 May 2023
EGAF00008003285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54044834
Sequences flagged as poor quality0
Sequence length102
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCGTCCCTAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27837175.15075501943442No Hit
ACCCGTCCCTAAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21322123.9452651478215293No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16010142.962381196323038No Hit
ACCCGTCCCTAAGAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15009072.777151651534354No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13399082.479252688610349No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10690211.978026243914451No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5989761.1082946429255385No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3647970.6749895836482724No Hit
GCACTCCTGCATGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3480170.6439412877093859No Hit
GCACTCCTGCATGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3366140.6228421388064583No Hit
ACCCGTCCCTAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3007770.5565323782842964No Hit
TCCTTTCCTGATAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2818490.5215096044147346No Hit
ACCCGTCCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2629330.48650903433249515No Hit
GCACTCCTGCATGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2495420.46173145799652193No Hit
TCAATCCTTCCGCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2455930.45442456165190553No Hit
CCTATTCCGATTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2333480.43176744700520314No Hit
ACCCGTCCCTAAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2312860.4279520962170038No Hit
ACCCGTCCCTAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2304790.4264588915195854No Hit
CCTATTCCGATTCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2180640.4034872232191517No Hit
TCCTTTCCTGATAGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2123720.39295522676598466No Hit
TCCTTTCCTGATAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2102460.3890214557787336No Hit
ACCCGTCCCTAAGAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1895250.3506810660201121No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1705340.31554172226710886No Hit
ACCCGTCCCTAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1669550.3089194426982605No Hit
TCAGGTCCTTTCAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1617390.29926819647554104No Hit
ACCCGTCCCTAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT1591660.2945073344105377No Hit
TGGATTCCCGGACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1574000.2912396770429529No Hit
GCATTCTGTCACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1566560.2898630422289761No Hit
CCTATTCCGATTCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1549770.2867563623194772No Hit
TGGATTCCCGGACTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1508240.2790720015903833No Hit
AGCTACGAGCAGTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1441910.26679885814803317No Hit
ACCCGTCCCTAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1402350.2594790095941455No Hit
TCAGGTCCTTTCAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1383320.25595785898796547No Hit
CCTATTCCGATTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1371330.2537393305713549No Hit
TCAGGTCCTTTCAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1361390.25190011685483205No Hit
CATTAACGGGATGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1351860.25013676607832674No Hit
ACCCGTCCCTAAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1343140.24852329086624633No Hit
TTCCGAGGATGATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1323420.24487446848296363No Hit
ACCCGTCCCTAAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT1306640.24176963888907493No Hit
CTTCACTCTGTCAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1301210.24076491751274506No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1287040.2381430202931144No Hit
CTTCACTCTGTCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1214850.22478559190319652No Hit
TTCCGAGGATGATCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1206760.22328868657455772No Hit
GGGTTGTTGTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1143790.2116372491772294No Hit
ACCCGTCCCTAAGAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT1121280.20747218873870535No Hit
ACCCGTCCCTAAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1101140.20374565309979487No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1099190.20338484155580902No Hit
ACCCGTCCCTAAGAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1098660.20328677482846927No Hit
AGCTACGAGCAGTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1093850.20239677301997078No Hit
CATTAACGGGATGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1084920.2007444411800765No Hit
ACCCGTCCCTAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA1024490.18956298394773494No Hit
GGGTTGTTGTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA957640.17719362409365527No Hit
GGGTTGTTGTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA936960.1733671714117949No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC918140.1698848774334287No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT917090.1696905942943594No Hit
CATTAACGGGATGCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA868070.1606203471732377No Hit
ACCCGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA864320.1599264788194187No Hit
ACCCGTCCCTAAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA827320.1530803110617381No Hit
ACCCGTCCCTAAGAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG826900.1530025978061104No Hit
CATTAGAGTCTGCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA799360.1479068286156638No Hit
TGGATTCCCGGACTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA782550.14479644807494457No Hit
AGCTACGAGCAGTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA777970.14394900352548035No Hit
CATTAGAGTCTGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750790.1389198456970004No Hit
CTTCACTCTGTCAGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA745980.13802984388850192No Hit
ACCCGTCCCTAAGAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA738680.13667911349306763No Hit
TTCCGAGGATGATCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA726510.13442727939547375No Hit
CATTAGAGTCTGCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA665640.1231644082762841No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG654100.12102914406213182No Hit
ACCCGTCCCTAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC651510.12054991231909419No Hit
GCGATCCCTTGAGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA644400.1192343379202534No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC636590.11778924142869973No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA610560.11297286989539093No Hit
ACCCGTCCCTAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACA607780.11245848215575979No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT606670.11225309712302937No Hit
ACCCGTCCCTAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG603590.1116831999150927No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT569390.10535512052826362No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG566970.1049073441505991No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT566900.10489439194132782No Hit
ACCCGTCCCTAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT566760.10486848752278524No Hit
GCGATCCCTTGAGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA566190.10476301953300476No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC563440.10425418274020418No Hit
ACCCGTCCCTAAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACC557590.10317174810824657No Hit
ACCCGTCCCTAAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTT547990.10139544512246998No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)