FastQCFastQC Report
Mon 22 May 2023
EGAF00008003303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences721697073
Sequences flagged as poor quality0
Sequence length102
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAGTTGCTGCAGTTAAAA18668510.2586751519220863Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9146020.12672934867230645No Hit
GTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGC8696420.12049958805915789No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGTGACTCTAGATAACCTCG8382950.11615607591635611Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)