FastQCFastQC Report
Wed 31 May 2023
EGAF00008003305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134475614
Sequences flagged as poor quality0
Sequence length102
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCGTCCCTAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49364443.6708841500437392No Hit
ACCCGTCCCTAAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34457852.5623865156696737No Hit
AGCTACGAGCAGTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29213582.1724072589101544No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25852191.9224444663996851No Hit
ACCCGTCCCTAAGAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24637261.8320987179132715No Hit
AGCTACGAGCAGTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23653041.7589092398566777No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22190241.6501311531472167No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17158081.2759250164122695No Hit
AGCTACGAGCAGTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17088521.270752331348344No Hit
CTTCACTCTGTCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14738811.0960210228153335No Hit
TAACTCAGGGCCTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13958551.0379986069444531No Hit
CTTCACTCTGTCAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12499250.9294807904725387No Hit
TAACTCAGGGCCTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12393040.9215827042068757No Hit
TAACTCAGGGCCTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9347580.6951133905958593No Hit
GCACTCCTGCATGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8902520.6620174271894382No Hit
CTTCACTCTGTCAGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8740460.6499661715617823No Hit
GCACTCCTGCATGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8091930.6017395838029042No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7764680.5774043165923005No Hit
CCTATTCCGATTCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6287800.4675791998986522No Hit
GCACTCCTGCATGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6166780.4585797987135422No Hit
CCTATTCCGATTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5729110.42603337732296953No Hit
TGAAGGCTCATTTGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5368490.39921661930467184No Hit
ACCCGTCCCTAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT5267300.3916918349225756No Hit
TGGATTCCCGGACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5048200.37539891805216075No Hit
TGGATTCCCGGACTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4891600.36375368399507735No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4366510.32470645569984163No Hit
TGGTAACGACAGTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4258610.3166826960909061No Hit
CCTATTCCGATTCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4189230.3115233963534831No Hit
ACCCGTCCCTAAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3697060.2749241955496853No Hit
GGGTTGTTGTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3641310.2707784624801936No Hit
TTCCGAGGATGATCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3517880.2615998466458015No Hit
TTCCGAGGATGATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3433640.2553355138426808No Hit
TCAATCCTTCCGCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3406910.25334779285707515No Hit
CATTAGAGTCTGCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3368970.2505264634820704No Hit
TCAGGTCCTTTCAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3213870.23899277381250703No Hit
GGGTTGTTGTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3120580.23205545653801588No Hit
ACCCGTCCCTAAGAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT3110130.23127836397162682No Hit
ACCCGTCCCTAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC3086250.22950257732230914No Hit
CATTAGAGTCTGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3018130.22443697487040287No Hit
GGGTTGTTGTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3009200.2237729139500341No Hit
CATTAACGGGATGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3002920.22330591478095055No Hit
GCGATCCCTTGAGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2895660.21532974744402356No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2791940.2076168248616437No Hit
CATTAGAGTCTGCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2790030.20747479167486826No Hit
AATCCTTCCGAATGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2784540.20706653921654522No Hit
TGAAGGCTCATTTGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2783270.20697209830177837No Hit
ACCCGTCCCTAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT2774520.20632142270791193No Hit
AATCCTTCCGAATGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2751020.20457389397009929No Hit
AGCTACGAGCAGTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2734890.2033744199896347No Hit
AGCTACGAGCAGTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2731690.20313645862959215No Hit
TGGTAACGACAGTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2730390.20303978682707485No Hit
TTTGTCCTGTACGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2643590.19658508493592006No Hit
TCAGGTCCTTTCAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2631840.19571132056701376No Hit
TCAGGTCCTTTCAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2594230.19291453095726338No Hit
CATTAACGGGATGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2513100.186881466850934No Hit
TGGATTCCCGGACTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2476750.18417837452670044No Hit
TGGTAACGACAGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2469980.18367493752436037No Hit
ACCGGATGACTGACCAAGAGGCTATTCAAGATCTCTGGCAGTGGAGGAAG2440220.18146189687596445No Hit
CCGGATGACTGACCAAGAGGCTATTCAAGATCTCTGGCAGTGGAGGAAGT2426100.1804118923747766No Hit
ACCCGTCCCTAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2414250.17953069171336894No Hit
TGAAGGCTCATTTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2381290.17707968970493043No Hit
CCTATTCCGATTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2346480.17449111628521735No Hit
ACCCGTCCCTAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2334260.17358240134155475No Hit
GCGATCCCTTGAGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2306880.1715463444546905No Hit
CATTAACGGGATGCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2281120.1696307555063478No Hit
TTCCGGATGACTGACCAAGAGGCTATTCAAGATCTCTGGCAGTGGAGGAA2264880.16842310160413174No Hit
CATCCGGATGACTGACCAAGAGGCTATTCAAGATCTCTGGCAGTGGAGGA2234750.16618254667348087No Hit
ACCCGTCCCTAAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC2221060.16516451823004877No Hit
GCAGAAATCTCCCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2132970.15861388816562683No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT2128330.1582688441935651No Hit
ACCCGTCCCTAAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT2100380.15619040043944327No Hit
ACCGGATGACTGACCAAGAGGCTATTCAAGATCTCTGTCTGGCAGTGGAG2092010.1555679827570819No Hit
CCGGATGACTGACCAAGAGGCTATTCAAGATCTCTGTCTGGCAGTGGAGG2079010.15460126473190894No Hit
TGTCCTACCCAACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1986440.14771748876342738No Hit
TTCCGGATGACTGACCAAGAGGCTATTCAAGATCTCTGTCTGGCAGTGGA1940510.14430199961756635No Hit
TTTGTCCTGTACGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1930770.14357770472793677No Hit
CATCCGGATGACTGACCAAGAGGCTATTCAAGATCTCTGTCTGGCAGTGG1928670.14342154258540882No Hit
ACCCGTCCCTAAGAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1907500.1418472794628772No Hit
ACCCGTCCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1900300.1413118664027814No Hit
AATCCTTCCGAATGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1900230.14130666099803046No Hit
TTCCGAGGATGATCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1889230.14048866882288413No Hit
CAATCAGACCTATGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1817030.1351196656369236No Hit
ACCCGTCCCTAAGAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT1809230.13453963482181983No Hit
GCAGAAATCTCCCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1797980.13370305191542015No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1748400.13001613809326054No Hit
ACCCGTCCCTAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATA1705710.1268415848244426No Hit
GCATTCTGTCACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1669800.12417121218721486No Hit
TGGCCTATAAATGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1666290.12391019832041815No Hit
AGCTACGAGCAGTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1655000.12307064089701795No Hit
TACGATTTGTCAGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1648570.12259248728918241No Hit
ACCCGTCCCTAAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG1646040.12240434908889875No Hit
CAGTAGATGGAGCATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1623000.12069102729659223No Hit
AGCTACGAGCAGTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1613070.11995260345121012No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1557060.11578753602121497No Hit
GCGATCCCTTGAGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1551600.11538151445064232No Hit
AGCTACGAGCAGTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT1549490.11522460867886426No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT1508770.11219655037232251No Hit
CTTCACTCTGTCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT1508750.11219506311382227No Hit
AGCTACGAGCAGTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1451070.1079058095990549No Hit
TACGATTTGTCAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1450880.10789168064330235No Hit
CAATCAGACCTATGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1433700.10661412559157381No Hit
AGCTACGAGCAGTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATT1414750.1052049481625717No Hit
CAGTAGATGGAGCATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1380690.10267214693661857No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)