FastQCFastQC Report
Mon 22 May 2023
EGAF00008003309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39400142
Sequences flagged as poor quality0
Sequence length102
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6267561.5907455358917233No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5903791.4984184574766253No Hit
GCACTCCTGCATGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5684421.4427409931669788No Hit
AGCTACGAGCAGTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5480151.3908960023545092No Hit
GCAGAAATCTCCCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4635281.1764627650326742No Hit
GCACTCCTGCATGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4594441.1660973201568665No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4516361.1462801326959684No Hit
GCAGAAATCTCCCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4378851.1113792432524736No Hit
ACCCGTCCCTAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4230191.0736484147696728No Hit
AGCTACGAGCAGTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4030781.0230369220496718No Hit
ACCCGTCCCTAAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3479720.8831744819599888No Hit
GCACTCCTGCATGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3249240.8246772308587111No Hit
AGCTACGAGCAGTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3233070.8205731847362377No Hit
GCAGAAATCTCCCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3216000.816240713041085No Hit
TTCCGAGGATGATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2688170.6822741907884494No Hit
ACCCGTCCCTAAGAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2325270.5901679237602747No Hit
TCGCTTGGATGGTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2227310.5653050692050805No Hit
TTCCGAGGATGATCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2090360.5305463112290306No Hit
TCGCTTGGATGGTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1869860.4745820459225756No Hit
TAACTCAGGGCCTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1804360.457957740355352No Hit
TCGCTTGGATGGTAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1765620.4481252884824628No Hit
TGGATTCCCGGACTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1629100.4134756671689153No Hit
TGGATTCCCGGACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1599950.40607721667602115No Hit
TAACTCAGGGCCTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1592240.4041203709367342No Hit
TGGTAACGACAGTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1438040.3649834561509956No Hit
TAACTCAGGGCCTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1353000.3433997775947102No Hit
TTCCGAGGATGATCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1188920.30175525763333544No Hit
CCTATTCCGATTCCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1128160.28633399341555676No Hit
TCAGGTCCTTTCAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1035750.26287976322521883No Hit
TCAGGTCCTTTCAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1023470.25976302318910427No Hit
GCATTCTGTCACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1020110.2589102343844345No Hit
CTTCACTCTGTCAGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1002970.2545599962558511No Hit
TGGTAACGACAGTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA914840.23219205656669967No Hit
TCAATCCTTCCGCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA905330.22977835968205396No Hit
GCACTCCTGCATGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA903780.2293849600846616No Hit
TGGTAACGACAGTCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA855660.2171718061320693No Hit
TCAGGTCCTTTCAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA833450.2115347706107252No Hit
CATTAACGGGATGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA816830.20731651170191215No Hit
CCTATTCCGATTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA806940.20480636846435732No Hit
CTTCACTCTGTCAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA798860.20275561443407997No Hit
TTCCGAGGATGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA798440.20264901583349626No Hit
TGGATTCCCGGACTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA769080.1951972660403102No Hit
TTCCGAGGATGATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA745730.1892708914602389No Hit
CATTAACGGGATGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA717220.18203487693013898No Hit
TCAATCCTTCCGCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA681890.17306790417151288No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT673260.17087755673570923No Hit
CTTCCGATTCATTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA668430.1696516728289964No Hit
TTCCGAGGATGATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA657790.16695117494754205No Hit
ACCCGTCCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA656440.16660853658852295No Hit
GGGTTGTTGTCTTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA652750.16567199174053737No Hit
GCACTCCTGCATGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT640250.16249941434221227No Hit
CCTATTCCGATTCCGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA634290.16098672943869086No Hit
CCTATTCCGATTCCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600520.1524156943393757No Hit
CTTCCGATTCATTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA579470.14707307400059624No Hit
GCATTCTGTCACCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT578430.14680911556105558No Hit
TTCCGAGGATGATCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA565620.143557858243252No Hit
GGGTTGTTGTCTTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA558420.14173045366181675No Hit
AGCTACGAGCAGTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT551960.14009086566236234No Hit
GGGTTGTTGTCTTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA543510.13794620334109456No Hit
TTTGTCCTGTACGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA529340.1343497696023532No Hit
GCAGAAATCTCCCTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT519950.1319665294607314No Hit
TTTGTCCTGTACGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510830.12965181699091338No Hit
GCGATCCCTTGAGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA504570.12806299022983217No Hit
GCACTCCTGCATGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT489870.12433203920940183No Hit
AGCTACGAGCAGTAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT487200.12365437667711959No Hit
CTTCACTCTGTCAGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA483110.12261630935238761No Hit
ACCCGTCCCTAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA481730.12226605680761252No Hit
GCATTCTGTCACCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT475910.12078890477095236No Hit
ACCCGTCCCTAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT469260.11910109359504338No Hit
GCGATCCCTTGAGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA459120.11652749880952207No Hit
GCACTCCTGCATGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC443160.11247675198734054No Hit
CATTAACGGGATGCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA437170.11095645289806316No Hit
GCAGAAATCTCCCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA437090.11093614840271387No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG431830.10960112783349868No Hit
AGCTACGAGCAGTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA426550.10826103114044613No Hit
CTTCCGATTCATTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA425440.10797930626747487No Hit
GCAGAAATCTCCCTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT401370.10187019122926003No Hit
GCACTCCTGCATGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT398940.10125344218302564No Hit
GCATTCTGTCACCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC395840.100466642988241No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)