FastQCFastQC Report
Wed 31 May 2023
EGAF00008003351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189208582
Sequences flagged as poor quality0
Sequence length114
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGACGTAGAAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8946100.4728168196937283No Hit
ACCTTTATGCCACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8677580.45862507441655054No Hit
CTACGACGTAGAAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8513480.44995210629505167No Hit
TTCTTTGCCTTGCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6767540.35767616502722904No Hit
CATTAGAGTCTGCCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6578770.34769934484261394No Hit
TAACTCAGGGCCTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6277240.3317629641133297No Hit
TAACTCAGGGCCTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6093990.3220778854523628No Hit
ACCTTTATGCCACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5879440.31073854778955No Hit
CATTAACAGCGCCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5666790.2994996284048046No Hit
TTCTTTGCCTTGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4812870.25436848313783145No Hit
ACCTTTATGCCACGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4748830.2509838586497096No Hit
TACGCCTATAACTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4639550.24520822210907958No Hit
CATTAGAGTCTGCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4101850.21678984941602703No Hit
TACGCCTATAACTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3923780.20737854269210687No Hit
CTACGACGTAGAAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3836640.2027730433495876No Hit
TAACTCAGGGCCTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3770880.19929751389395223No Hit
TTCTTTGCCTTGCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3447970.18223116327778408No Hit
CATTAGAGTCTGCCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3241760.17133260900396155No Hit
GTATTCCCTCAGTCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3228650.17063972288529702No Hit
GCGCTACTTCCTTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3216000.16997114856026985No Hit
CATTAACGGGATGCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3005570.15884956000568728No Hit
TGTACCCGCTTGTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3001240.15862071203514438No Hit
TGGATTCCCGGACTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2948220.15581851355981305No Hit
CATTAACGGGATGCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2917600.1542001937311702No Hit
GTATTCCCTCAGTCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2863810.15135729942735895No Hit
TTCTTTGCCTTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2817030.14888489571789085No Hit
GCTGCGCTTTCCATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2802330.14810797535600156No Hit
TGGATTCCCGGACTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2684340.14187200028802077No Hit
GCTGCGCTTTCCATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2661210.14064953988186435No Hit
TACGCCTATAACTTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2646670.13988107579602282No Hit
GCGCTACTTCCTTGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2333460.12332738691525101No Hit
GTATTCCCTCAGTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2298940.12150294535794365No Hit
TGGATTCCCGGACTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2227120.11770713444699882No Hit
TGTACCCGCTTGTGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2215500.11709299739902919No Hit
TGGCTTCAGGTCCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2118720.11197800742463151No Hit
CATTAGAGTCTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2107120.11136492741116784No Hit
GCGCTACTTCCTTGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2005540.10599624915533694No Hit
TGGCTTCAGGTCCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1999090.10565535552716102No Hit
GCTGCGCTTTCCATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1996620.10552481176567349No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)