FastQCFastQC Report
Thu 1 Jun 2023
EGAF00008003375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449284647
Sequences flagged as poor quality0
Sequence length202
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA22698460.5052133464066489No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT21904920.4875510468978923No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA21569860.48009341391983956No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA17149350.3817034504631092No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG14735200.327970254456525No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC14068450.3131299966277281No Hit
CCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACT11681180.2599950850312497No Hit
GAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAAC11276640.2509909936895751No Hit
CCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCAC10802120.24042931518200752No Hit
GTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACAT10395900.2313878310647014No Hit
CCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTA10058420.22387633468365548No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG9938220.2212009706176316No Hit
GTCCTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATG9646610.21471043055695602No Hit
GGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTC9301280.20702421198025045No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC9299160.2069770258586201No Hit
CCCTATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATA9161000.20390191521501067No Hit
ATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATATCCA9080010.20209927182310328No Hit
TCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCAT8648010.19248398666068817No Hit
CCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGC8251570.18366018191580893No Hit
ATTATACCCTAGCCAACCCCTTAAACACCCCTCCCCACATCAAGCCCGAA8062250.1794463722237987No Hit
GTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAAT7644400.1701460321656618No Hit
CCATATATCCAAACAACAAAGCATAATATTTCGCCCACTAAGCCAATCAC7599860.16915467845933316No Hit
CTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCAC7215630.16060263906591937No Hit
ATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGG7101150.15805458849787937No Hit
GGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATT6955030.15480230732211067No Hit
CCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTC6868280.1528714601280377No Hit
CCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGC6759660.1504538391226175No Hit
GTCTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTC6717680.149519464883918No Hit
TTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTA6418510.14286065733290015No Hit
TCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTC6398890.14242396313177377No Hit
CCATAAACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTC6284980.1398885993983231No Hit
GCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGG6237880.13884026622436532No Hit
CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC5770290.12843283291627813No Hit
GTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATGCCCCCCACC5695760.1267739736497161No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT5615210.12498112360380745No Hit
GACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTC5528910.12306029233177869No Hit
CTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCAT5526380.12300398059228584No Hit
GGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTG5487700.12214305644857701No Hit
GACTATATGGATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATAC5478650.12194162512746624No Hit
GTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCA5469350.1217346294052198No Hit
TCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTC5334350.1187298527919651No Hit
CTATATGGATGCCCCCCACCCTACCACACATTCGAAGAACCCGTATACAT5332010.11867776999733533No Hit
CAATTATACCCTAGCCAACCCCTTAAACACCCCTCCCCACATCAAGCCCG5206070.11587464728123682No Hit
CTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCATG5129140.1141623697637725No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC5072700.1129061505633866No Hit
CTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGCT5045600.1123029694802814No Hit
CCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCTAACAGCA5043940.11226602185674063No Hit
GTACTACGTTGTAGCCCACTTCCACTATGTCCTATCAATAGGAGCTGTAT5038580.11214672109639216No Hit
ACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTC5031900.11199804029804739No Hit
CCCTACCACACATTCGAAGAACCCGTATACATAAAATCTAGACAAAAAAG4926090.10964296316139199No Hit
GCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTCT4904860.109170434216952No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT4813910.10714610508380001No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA4753830.10580886820287895No Hit
ATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGA4625370.10294965632333304No Hit
TCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATA4582120.10198701492686439No Hit
CTTTATTGACTCCTAGCCGCAGACCTCCTCATTCTAACCTGAATCGGAGG4562370.10154742723714749No Hit
GATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTAC4555820.10140163992739329No Hit
GCATAATATTTCGCCCACTAAGCCAATCACTTTATTGACTCCTAGCCGCA4549620.101263642779229No Hit
CACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCT4542490.10110494605884007No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)