FastQCFastQC Report
Mon 22 May 2023
EGAF00008003389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440294723
Sequences flagged as poor quality0
Sequence length202
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA31393000.7129996877114514No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT28587870.6492894079041688No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA28483700.6469234926533517No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA21908490.4975869311065988No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC19291390.43814719987002887No Hit
CCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACT19209920.4362968483726297No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG19140460.43471926871129No Hit
GAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAAC13608400.30907479215916017No Hit
CCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTA13485430.30628189018745067No Hit
GGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTC13021060.295735091060812No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC12693080.2882859897460093No Hit
CCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCAC12656200.2874483689871523No Hit
GTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACAT12208110.27727132219115874No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG11780820.2675666862353015No Hit
GTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAAT10684810.24267404176906296No Hit
CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC10509140.23868421425527736No Hit
CCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGC10391560.23601373028493008No Hit
ATTATACCCTAGCCAACCCCTTAAACACCCCTCCCCACATCAAGCCCGAA9356350.21250197904370524No Hit
CCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTC9261990.21035886909777932No Hit
CCCTATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATA9110910.20692753113009713No Hit
ATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATATCCA8800600.19987975190881407No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA8502830.19311678191519002No Hit
TTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTA8399180.19076267693537632No Hit
GTCTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTC8232580.18697884780236168No Hit
GCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGG7649120.17372726949534664No Hit
GATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTAC7598220.17257122566059008No Hit
GTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCA7311190.16605218318730566No Hit
CTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCAC7002010.15903006859339533No Hit
CTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCAT6985890.15866395019228974No Hit
TCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTC6942580.15768029089006366No Hit
TCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATA6881230.15628690603225784No Hit
CCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAAC6761690.15357190642505159No Hit
TCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTC6742090.1531267500564616No Hit
CAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAA6726890.15278152675020817No Hit
CACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTA6597880.14985144393838215No Hit
CCATATATCCAAACAACAAAGCATAATATTTCGCCCACTAAGCCAATCAC6499720.14762202816589287No Hit
ATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTA6413400.14566152317932732No Hit
CCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGC6189930.14058605921561318No Hit
CACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCT6177120.14029511773185616No Hit
CTCCAGCCTAGCCCCTACCCCCCAATTAGGAGGGCACTGGCCCCCAACAG6161180.1399330875014825No Hit
ATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAA6088460.13828146652577528No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG6085230.13820810657319643No Hit
TCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCAT6028640.13692283111919104No Hit
CTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTA5948710.13510745619360967No Hit
ATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGA5922100.13450308828706994No Hit
GGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTG5815540.13208289121375638No Hit
ATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGG5716760.1298393939643015No Hit
CACACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCCTATTTATTA5711600.1297221997366523No Hit
CAATTATACCCTAGCCAACCCCTTAAACACCCCTCCCCACATCAAGCCCG5683260.12907853996696664No Hit
CCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGC5509900.1251411773109077No Hit
GGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATT5509650.12513549929600223No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTACGACTCCTT5463650.12409074455339317No Hit
TCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATC5462490.12406439856423171No Hit
ATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCT5389660.12241027926196608No Hit
CTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTT5145610.11686740111123248No Hit
CATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGT5023340.1140903975812583No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT4970750.11289597036574067No Hit
GGTCTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTT4970290.11288552281831457No Hit
CCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACGC4906430.11143513069085773No Hit
GAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCA4900430.1112988583331261No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC4863490.11045987485069177No Hit
CCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCA4843370.1100029082110984No Hit
GTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTAT4772470.10839262318390311No Hit
ATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTCTGAGCCTTTT4707230.10691088841416799No Hit
GCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACT4678930.10626813712686718No Hit
GCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATT4669380.10605123695747769No Hit
CTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGG4623860.10501738400348715No Hit
CCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAA4539920.1031109337188218No Hit
GGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCACGGACTTCA4502340.10225741451822942No Hit
ATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAA4494660.10208298590033293No Hit
ATTCTCACCAGACCTCCTAGGCGACCCAGACAATTATACCCTAGCCAACC4492520.102034382092742No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC4467060.1014561330547675No Hit
ATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGT4407050.10009318235685509No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)