FastQCFastQC Report
Thu 1 Jun 2023
EGAF00008003391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences534299604
Sequences flagged as poor quality0
Sequence length202
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG27876270.5217348055530283No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA23451980.4389293913831911No Hit
CCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACT20456610.38286777393905763No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC16692970.312427145276342No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA16641970.3114726246362705No Hit
CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC16452210.3079210592115655No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT16060580.3005912765003659No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG12512640.23418770866242303No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA12382680.23175536547842923No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA12164390.22766982997801363No Hit
GGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTC11528110.21576115560811832No Hit
ATTATACCCTAGCCAACCCCTTAAACACCCCTCCCCACATCAAGCCCGAA10977310.20545233269534674No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC10853920.20314295422910328No Hit
CCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTA10304390.19285790075187853No Hit
CAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAA10270830.19222978873852956No Hit
GATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTAC10096410.1889653281494852No Hit
GTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAAT9679380.18116015672734806No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT9589710.17948188484901068No Hit
ATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTA8679350.16244350426282553No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG8443680.15803268310114638No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG8370370.1566606064712711No Hit
CCCTATTACTATCCATCCTCATCCTAGCAATAATTCCCATCCTCCATATA8301310.1553680732280685No Hit
CAATTATACCCTAGCCAACCCCTTAAACACCCCTCCCCACATCAAGCCCG8122060.15201321391958209No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC7995230.14963945210036128No Hit
CCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTC7918090.14819569284202574No Hit
GGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATT7813950.14624659912718183No Hit
GAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAAC7719390.1444768055639435No Hit
CCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAAC7576350.1417996559099078No Hit
CACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTA7264330.13595986120176876No Hit
CCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGC7183370.13444460647588277No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC7165630.13411258302186577No Hit
ATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGG7017900.13134765490112546No Hit
CTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTA6901030.12916030534808332No Hit
ATTACTATCCATCCTCATCCTAGCAATAATTCCCATCCTCCATATATCCA6784940.12698755434600698No Hit
GTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACAT6576060.12307813726173003No Hit
CCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCAC6414540.12005511424635082No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6365490.11913708998369386No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC6138210.11488329682535195No Hit
GTACTACGTTGTAGCCCACTTCCACTATGTCCTATCAATAGGAGCTGTAT6116960.11448557989198883No Hit
TCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATA6041280.11306914612648675No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT6024030.1127462935570508No Hit
CCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGC6008760.11246049884775884No Hit
GTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCA5986980.11205286238617537No Hit
CCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGC5871480.10989115387777827No Hit
TCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATC5720310.10706184240406062No Hit
CACACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCCTATTTATTA5666630.10605716264015796No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA5555520.10397761777117095No Hit
CTCCAGCCTAGCCCCTACCCCCCAATTAGGAGGGCACTGGCCCCCAACAG5525230.10341070737533245No Hit
TTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTA5487600.10270642087168756No Hit
CCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGA5388180.1008456671062777No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)