FastQCFastQC Report
Thu 1 Jun 2023
EGAF00008003397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences543673399
Sequences flagged as poor quality0
Sequence length202
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA33527930.6166924859974618No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA31473300.5789008632368272No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT29745060.547112660923107No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA24019840.441806423565704No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC22512130.414074516822185No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG21318190.3921139058709032No Hit
GAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAAC17000190.3126912229156167No Hit
ATTATACCCTAGCCAACCCCTTAAACACCCCTCCCCACATCAAGCCCGAA16190740.297802688705761No Hit
CCCTATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATA15980440.2939345575743352No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG15914060.29271360396280854No Hit
CCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTA15286300.2811669658312637No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC14840360.2729646149194804No Hit
CCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCAC14717290.2707009397014843No Hit
GTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACAT14375850.26442069864816026No Hit
ATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATATCCA14261430.2623161263036156No Hit
GGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTC13213260.24303672065441626No Hit
GTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAAT12703570.23366179076199384No Hit
CAATTATACCCTAGCCAACCCCTTAAACACCCCTCCCCACATCAAGCCCG12217090.22471377158550296No Hit
CCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGC12078580.222166102336745No Hit
CCATATATCCAAACAACAAAGCATAATATTTCGCCCACTAAGCCAATCAC11412360.209912054203704No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT10766050.1980242185805379No Hit
CCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTC10432280.1918850548728061No Hit
GTCTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTC9402920.17295162899812944No Hit
GGCTACACCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATT9199500.16921004442963375No Hit
TTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTA9011650.1657548450333506No Hit
ATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGG8929120.1642368380800621No Hit
GTCCTAATAGTAGAAGAACCCTCCATAAACCTGGAGTGACTATATGGATG8853180.16284004360492907No Hit
CTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCAC8819620.1622227612427291No Hit
TCTGTAGGCTCATTCATTTCTCTAACAGCAGTAATATTAATAATTTTCAT8816660.1621683167912359No Hit
GCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGG8791290.161701676340431No Hit
CCCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGC8450560.15543449459810704No Hit
GTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCA8351690.15361593955785943No Hit
GGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTG7747850.14250927145324613No Hit
TCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTC7536500.13862182725625685No Hit
CTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCAT7528520.1384750479579745No Hit
TCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTC7274220.13379760741246052No Hit
ATTCTCACCAGACCTCCTAGGCGACCCAGACAATTATACCCTAGCCAACC7235560.13308651873180943No Hit
CACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCT7084030.13029936747006451No Hit
CATTAACACTATTCTCACCAGACCTCCTAGGCGACCCAGACAATTATACC7026290.12923733279803154No Hit
ATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGA6834910.1257172047146636No Hit
GACTACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTC6830060.12562799674515618No Hit
CACACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCCTATTTATTA6795600.12499416032675896No Hit
CCATATACCAATGATGGCGCGATGTAACACGAGAAAGCACATACCAAGGC6750610.12416664145085383No Hit
CTCCAGCCTAGCCCCTACCCCCCAATTAGGAGGGCACTGGCCCCCAACAG6718310.12357253476733006No Hit
ATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAA6649460.12230614946823984No Hit
GTACTACGTTGTAGCCCACTTCCACTATGTCCTATCAATAGGAGCTGTAT6584180.12110542859206544No Hit
CCATAAACCTGGAGTGACTATATGGATGCCCCCCACCCTACCACACATTC6473880.11907663703811266No Hit
GCATAATATTTCGCCCACTAAGCCAATCACTTTATTGACTCCTAGCCGCA6413430.11796475626353019No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT6385740.11745544313452791No Hit
CATCAAGCCCGAATGATATTTCCTATTCGCCTACACAATTCTCCGATCCG6362080.11702025539049779No Hit
CTTTATTGACTCCTAGCCGCAGACCTCCTCATTCTAACCTGAATCGGAGG6286270.11562585205681546No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6175350.11358565659748233No Hit
CTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTT6170540.1134971843638059No Hit
TCCTAGCAATAATCCCCATCCTCCATATATCCAAACAACAAAGCATAATA6067560.11160303246692414No Hit
TCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTA6066780.11158868561821984No Hit
ATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCT6001650.11039072375141165No Hit
CATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGT5998200.11032726653598883No Hit
GGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCACGGACTTCA5976930.10993603900786031No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC5967690.1097660840309018No Hit
ACCCCGATGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTC5759480.10593639509664514No Hit
GGTCTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTT5706750.10496651133744361No Hit
GCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACT5678030.10443825301079335No Hit
ATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGT5638900.10371851943412813No Hit
GAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCA5607840.10314722056136501No Hit
CTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGG5569740.10244643218234777No Hit
CTTAAACACCCCTCCCCACATCAAGCCCGAATGATATTTCCTATTCGCCT5508630.10132241176655399No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG5507530.10130217903120178No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)