FastQCFastQC Report
Mon 22 May 2023
EGAF00008003405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149581876
Sequences flagged as poor quality0
Sequence length130
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA11879180.794159046380726No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT11120610.7434463517491918No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA10770290.7200264021290922No Hit
CCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACT10198290.6817864752545288No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA8551080.5716655138086382No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG6946970.464425917482142No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC5228820.3495624028675774No Hit
GAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAAC5187380.34679201375974184No Hit
CCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTA5061740.3383926004511402No Hit
GATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTAC4982960.33312591961341625No Hit
GTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACAT4944000.3305213259927292No Hit
CCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAAC4687300.3133601560124838No Hit
CCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCAC4677220.3126862775808481No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG4277190.285943064385688No Hit
CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC4233420.2830169077435558No Hit
CCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGC3822180.25552427220527707No Hit
TCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATA3681880.2461447936379672No Hit
ATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATATCCA3613690.2415860862715748No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC3578460.2392308544118005No Hit
CTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCAC3552310.2374826479646505No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA3522080.2354616811999336No Hit
CACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTA3520330.23534468841666353No Hit
GTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAAT3466620.2317540127655572No Hit
CATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAG3449490.23060882054989068No Hit
CCATATATCCAAACAACAAAGCATAATATTTCGCCCACTAAGCCAATCAC3430550.22934262436981337No Hit
CTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTA3375640.2256717250958933No Hit
CCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCA3332030.22275626493680292No Hit
ATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTA3279960.21927522823687542No Hit
ACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACC3269740.21859199038257818No Hit
CCCTATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATA3194630.2135706601246263No Hit
TCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATC3180720.21264073463017671No Hit
CATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAG3098920.20717215767503813No Hit
CAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTA3066670.20501614781191804No Hit
GCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATT2989160.19983437030833867No Hit
CCTAATTGGAAGCGCCACCCTAGCAATATCAACCATTAACCTTCCCTCTA2961220.1979664969571581No Hit
TTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTA2880730.19258549745692452No Hit
GCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCAT2849580.1905030259147171No Hit
CCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTC2841990.18999561150042002No Hit
GCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGG2817520.18835971812520924No Hit
GTCTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTC2799390.1871476728905312No Hit
TCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTC2693640.18007796613006777No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC2675320.17885321882177757No Hit
CCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAA2669500.1784641342511308No Hit
GGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTG2647300.1769799972290761No Hit
CCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCT2520140.16847896733157697No Hit
AGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCG2512480.1679668732059491No Hit
CTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCAT2488420.16635838956853302No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC2481740.16591181140153638No Hit
CACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCT2475590.1655006653346158No Hit
TCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTC2474180.1654064025778096No Hit
CTCATGCACCTAATTGGAAGCGCCACCCTAGCAATATCAACCATTAACCT2464320.1647472318103565No Hit
CCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAAT2464270.1647438891594059No Hit
GCATAATATTTCGCCCACTAAGCCAATCACTTTATTGACTCCTAGCCGCA2440090.16312738315970848No Hit
ATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGA2405620.16082295959438295No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG2394690.16009225609658753No Hit
GTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCA2363610.15801446426571092No Hit
CAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAA2213570.14798383729322928No Hit
ATTATACCCTAGCCAACCCCTTAAACACCCCTCCCCACATCAAGCCCGAA2213380.1479711352196171No Hit
CCATTAACCTTCCCTCTACACTTATCATCTTCACAATTCTAATTCTACTG2210160.14775586849840017No Hit
TCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCAACTAATA2125530.1420980974994591No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA2123170.14194032437459203No Hit
ACATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGTCATTATTGGCTC2122540.1418982069726148No Hit
ATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAA2101600.14049830475451452No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG2082160.13919868206493147No Hit
CTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTT2070610.1384265296953489No Hit
TCCTAGCAATAATCCCCATCCTCCATATATCCAAACAACAAAGCATAATA2058000.13758351312561423No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT2055650.13742640853093727No Hit
CTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGG2049600.13702194776591786No Hit
CCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCC2018690.13495552094827315No Hit
ATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCACGGACTTCACGT1986920.13283160053427862No Hit
CTTTATTGACTCCTAGCCGCAGACCTCCTCATTCTAACCTGAATCGGAGG1930550.1290630958526018No Hit
GGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCTTCCACGGACTTCA1883090.12589025157031725No Hit
GACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGC1871560.12511943626111494No Hit
CCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAAC1865910.12474171670370013No Hit
CAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTC1861420.1244415466483386No Hit
CACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCT1745980.116724034133654No Hit
TACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTG1744920.11665316993350185No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC1744530.11662709725608737No Hit
CCCTAGCAATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACA1718990.11491967115053431No Hit
GCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGT1702560.11382127604817578No Hit
ATTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCT1682100.11245346327920101No Hit
GGTCTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTT1664940.11130626547296411No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG1658490.11087506350034011No Hit
ATATCAACCATTAACCTTCCCTCTACACTTATCATCTTCACAATTCTAAT1657990.11084163699083437No Hit
GGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTC1653240.11052408515052987No Hit
CATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGT1644870.10996452538140383No Hit
GCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACT1637040.10944106624254399No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC1617530.10813676384163011No Hit
CTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTGCTTCATCCGCCA1610690.10767948919159163No Hit
GGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAA1585430.1059907819313618No Hit
GTACTATACTTCACAACAATCCTAATCCTAATACCAACTATCTCCCTAAT1581010.10569529158733107No Hit
GATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCC1572010.10509361441622779No Hit
CCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAA1561070.10436224238824228No Hit
GTCTTGCACTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAAT1560990.10435689414672136No Hit
TCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACAATCGAGTAGTA1545870.10334607649926787No Hit
GAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCA1541890.1030800014836022No Hit
AGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCA1538340.10284267326611145No Hit
CCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAA1514960.10127964968162319No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA1500860.10033702211356141No Hit
AGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCT1498700.10019261959249662No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)