FastQCFastQC Report
Mon 22 May 2023
EGAF00008003407

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003407
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255602384
Sequences flagged as poor quality0
Sequence length202
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACT15166970.5933813981954097No Hit
CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC12921970.505549666547711No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC10208380.39938516379409045No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA10095680.39497597174211024No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG9890290.38694044418615436No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA9451550.36977550256338765No Hit
ATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATT8354890.3268705819269667No Hit
CTATTTTACCCTCCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCA7737030.3026978809399524No Hit
GATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTAC7639100.2988665395233559No Hit
CCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAAAT7619730.2981087218654424No Hit
CCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAAC7510370.2938302015211251No Hit
CACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTA7498540.29336737328709733No Hit
CCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACGATTAACC7242710.28335846820583643No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC6638280.2597111926780777No Hit
GTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCT6264830.2451006090772612No Hit
CCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTA6109950.23904119767521417No Hit
CCTACAAGCCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCA6044360.23647510267353375No Hit
CTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCAC5867520.22955654435523576No Hit
TCCCCGTTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAGGGACA5655280.2212530224287736No Hit
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG5505110.21537788160849078No Hit
CTCCTGCCTCACTCATTTACACCAACCACCCAACTATCTATAAACCTAGC5367570.20999686763484962No Hit
CTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTA5335680.20874922668952883No Hit
CTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATA5241360.20505912026235248No Hit
CAACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAA5053880.19772429039628991No Hit
GAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAAC4786570.18726625022401983No Hit
CCTACATACTTCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTT4692390.1835816210540509No Hit
ATCTATAAACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTA4661260.1823637137907133No Hit
GTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACAT4582780.17929331989329178No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC4528470.17716853532946703No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG4505010.176250703514565No Hit
CTCTAAATCACCACGATCAAAAGGGACAAGCATCAAGCACGCAGCAATGC4498720.1760046181728884No Hit
ACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTT4495180.175866121812072No Hit
GCCCACTTCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGT4351570.170247629615223No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4344180.16995850868120227No Hit
GTATTACTCGCATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAAT4319180.16898042703701854No Hit
GCCTAGCCCCTACCCCCCAATTAGGAGGGCACTGGCCCCCAACAGGCATC4312980.16873786278926098No Hit
TCTTACCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATC4309240.16859154177529112No Hit
CCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCA4256210.16651683499164857No Hit
CTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCT4189840.16392022384266963No Hit
GGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGA4187880.16384354224176564No Hit
ATTATACCCTAGCCAACCCCTTAAACACCCCTCCCCACATCAAGCCCGAA4067950.15915148897828746No Hit
CCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCAC4042460.15815423693387776No Hit
CATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAG4007390.1567821840034168No Hit
CCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGCTC3963160.15505176195852696No Hit
GTTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAGGGACAAGCAT3961640.1549922945945606No Hit
AAGCTATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTC3916600.1532301827043992No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAAC3884710.15198254175907844No Hit
GGATAATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTC3846130.1504731661657741No Hit
ACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTT3838260.15016526606418507No Hit
GCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGAT3774050.14765316116926358No Hit
TCCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATA3742870.14643329774263764No Hit
ACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACC3720180.14554559084237648No Hit
CACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCT3651300.14285078029632148No Hit
CCACAAGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAAC3579160.14002842790386494No Hit
CAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTA3430140.1341982788392146No Hit
GCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAGGCCACCTACTCAT3418220.13373192951126778No Hit
GCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATT3379610.13222138021999044No Hit
CAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACA3338240.1306028507151952No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3289550.128697938904983No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG3277690.12823393697298222No Hit
CCTCTACACTTATCATCTTCACAATTCTAATTCTACTGACTATCCTAGAA3238700.1267085208407133No Hit
CCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCATAACGC3238620.1267053909794519No Hit
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC3231070.1264100103229084No Hit
GTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCA3227050.12625273479452367No Hit
CCCTATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATA3224230.12614240718505976No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG3153120.12336035175634355No Hit
CAATTATACCCTAGCCAACCCCTTAAACACCCCTCCCCACATCAAGCCCG3123320.12219447843647656No Hit
ATCCTATTTATTACCTCAGAAGTTTTTTTCTTCGCAGGATTTTTCTGAGC3109180.12164127545852624No Hit
CCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCT3070240.12011781548954567No Hit
CATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTACTGCAG3045300.11914208124130798No Hit
GGCTTCCACGGACTTCACGTCATTATTGGCTCAACTTTCCTCACTATCTG3043220.11906070484851189No Hit
AGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCT3010150.11776689844958566No Hit
CCCCTTATGAGCGGGCGCAGTGATTATAGGCTTTCGCTCTAAGATTAAAA2914050.11400715260934344No Hit
CTCCAGCCTAGCCCCTACCCCCCAATTAGGAGGGCACTGGCCCCCAACAG2906620.11371646674469202No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTG2905100.11365699938072565No Hit
CCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGC2904810.11364565363365313No Hit
CACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAACCAAATAATTC2876380.11253337918788739No Hit
CGATTAACCCAAGTCAATAGAAGCCGGCGTAAAGAGTGTTTTAGATCACC2847450.11140154310923797No Hit
CTATATCCCCCGCCCGCGTCCCTTTCTCCATAAAATTCTTCTTAGTAGCT2839590.11109403424030662No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT2831670.11078417797542922No Hit
ATACTAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACACG2787190.10904397511409752No Hit
CATCAAGCCCGAATGATATTTCCTATTCGCCTACACAATTCTCCGATCCG2751770.10765822904061802No Hit
CTATCTGCTTCATCCGCCAACTAATATTTCACTTTACATCCAAACATCAC2746040.10743405272777111No Hit
CCTTTTACCCCTACCATGAGCCCTACAAACAACTAACCTGCCACTAATAG2724560.10659368497908846No Hit
GTGATTATAGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTT2724280.1065827304646736No Hit
CCGTTCCAGTGAGTTCACCCTCTAAATCACCACGATCAAAAGGGACAAGC2723700.10656003897052853No Hit
ACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTTT2679780.10484174513802656No Hit
CCATATACCAATGATGACGCGATGTAACACGAGAAAGCACATACCAAGGC2653020.1037948065460923No Hit
CTCATGCACCTAATTGGAAGCGCCACCCTAGCAATATCAACCATTAACCT2639980.10328463916048608No Hit
AACCTAGCCATGGCCATCCCCTTATGAGCGGGCGCAGTGATTATAGGCTT2629700.10288245198839772No Hit
ATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATATCCA2620350.10251664945347301No Hit
AGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCG2611510.10217079978408965No Hit
CCCTAGAAGTCCCACTCCTAAACACATCCGTATTACTCGCATCAGGAGTA2609270.10208316366877078No Hit
GGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAACCGAAA2596800.10159529654465195No Hit
GATTAAAAATGCCCTAGCCCACTTCTTACCACAAGGCACACCTACACCCC2595330.10153778534397395No Hit
ACTAACCTCCTCGGACTCCTGCCTCACTCATTTACACCAACCACCCAACT2559310.10012856531103403No Hit
ATGTAACACGAGAAAGCACATACCAAGGCCACCACACACCACCTGTCCAA2558850.10011056860878105No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)