FastQCFastQC Report
Wed 31 May 2023
EGAF00008003433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14036305
Sequences flagged as poor quality0
Sequence length130
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCAGCAGCAGCAGCAGAGCGAGCTGCAGCCCCCGGCGCCCAGCGAGGA152866310.890779304097482No Hit
ATACCAGCAGCAGCAGCAGAGCGAGCTGCAGCCCCCGGCGCCCAGCGAGG13883639.891228496388473No Hit
CATTACCAGCAGCAGCAGCAGAGCGAGCTGCAGCCCCCGGCGCCCAGCGA11306118.054904763041272No Hit
TTTGATCAGGCTACCCAGGGCAACATCAGCAGTGACCGAGGAAAGAAAAG11019347.850598857747819No Hit
TTTACCAGCAGCAGCAGCAGAGCGAGCTGCAGCCCCCGGCGCCCAGCGAG10204977.270410553204708No Hit
CATTGATCAGGCTACCCAGGGCAACATCAGCAGTGACCGAGGAAAGAAAA8985976.4019483760149125No Hit
TGATCAGGCTACCCAGGGCAACATCAGCAGTGACCGAGGAAAGAAAAGAA7838935.58475325236948No Hit
ATGATCAGGCTACCCAGGGCAACATCAGCAGTGACCGAGGAAAGAAAAGA7042355.017239223570591No Hit
TGATCAGGCTACCCAGGGCAACATCAGCAGTGACCGAGGAAAGAAAAGTA6273814.469701962161694No Hit
ATGATCAGGCTACCCAGGGCAACATCAGCAGTGACCGAGGAAAGAAAAGT5611163.997604782740187No Hit
TGATCAGGCTACCCAGGGCAACATCAGCAGTGACCGAGGAAAAAAAAAAA818070.5828243259176827No Hit
ATGATCAGGCTACCCAGGGCAACATCAGCAGTGACCGAGGAAAAAAAAAA753800.5370359222031724No Hit
CATTACCAGCAGCAGCAGCAGCAACAGTTTGTGTAGCCAGTCTACCACCA726400.517515115267159No Hit
TACCAGCAGCAGCAGCAGCAACAGTTTGTGTAGCCAGTCTACCACCACCA680600.48488544527922406No Hit
TTTGATCAGGCTACCCAGGGCAACATCAGCAGTGACCGAGGAAAAAAAAA665800.4743413597809395No Hit
TGATCAGGCTACCCAGGGCAACATCAGCAGTGACCGAAAAAAAAAAAAAA647630.4613963575171671No Hit
ATACCAGCAGCAGCAGCAGCAACAGTTTGTGTAGCCAGTCTACCACCACC603200.42974272787603296No Hit
ATGATCAGGCTACCCAGGGCAACATCAGCAGTGACCGAAAAAAAAAAAAA587530.4185788211356194No Hit
TTTACCAGCAGCAGCAGCAGCAACAGTTTGTGTAGCCAGTCTACCACCAC565320.402755568506099No Hit
CATTGATCAGGCTACCCAGGGCAACATCAGCAGTGACCGAGGAAAAAAAA557230.3969919433925096No Hit
TGATCAGGCTACCCAGGGCAACATCAGCAGTGACCAAAAAAAAAAAAAAA549600.39155603985521825No Hit
TTTGATCAGGCTACCCAGGGCAACATCAGCAGTGACCGAAAAAAAAAAAA530010.37759937533417803No Hit
ATGATCAGGCTACCCAGGGCAACATCAGCAGTGACCAAAAAAAAAAAAAA500100.3562903484927123No Hit
TTTGATCAGGCTACCCAGGGCAACATCAGCAGTGACCAAAAAAAAAAAAA450680.3210816521869538No Hit
CATTGATCAGGCTACCCAGGGCAACATCAGCAGTGACCGAAAAAAAAAAA436850.3112286317517324No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG394380.28097138100091157No Hit
CATTGATCAGGCTACCCAGGGCAACATCAGCAGTGACCAAAAAAAAAAAA377310.2688100607674171No Hit
TGATCAGGCTACCCAGGGCAACATCAGCAGTGACCGAGAAAAAAAAAAAA351960.2507497521605579No Hit
CATTACCAGCAGCAGCAGCAACAGTTTGTGTAGCCAGTCTACCACCACCA344790.24564157019956465No Hit
ATGATCAGGCTACCCAGGGCAACATCAGCAGTGACCGAGAAAAAAAAAAA326390.23253270714764318No Hit
TACCAGCAGCAGCAGCAACAGTTTGTGTAGCCAGTCTACCACCACCACAG307080.21877552532521913No Hit
TTTGATCAGGCTACCCAGGGCAACATCAGCAGTGACCGAGAAAAAAAAAA292150.20813882285971985No Hit
TTTACCAGCAGCAGCAGCAACAGTTTGTGTAGCCAGTCTACCACCACCAC267440.1905344747068406No Hit
ATACCAGCAGCAGCAGCAACAGTTTGTGTAGCCAGTCTACCACCACCACA264740.18861089154161298No Hit
CATTGATCAGGCTACCCAGGGCAACATCAGCAGTGACCGAGAAAAAAAAA241430.1720039568818147No Hit
TACCAGCAGCAGCAGAGCGAGCTGCAGCCCCCGGCGCCCAGCGAGGATAT158050.11260085898674901No Hit
ATACCAGCAGCAGCAGAGCGAGCTGCAGCCCCCGGCGCCCAGCGAGGATA156970.11183142572065796No Hit
CATTACCAGCAGCAGCAGAGCGAGCTGCAGCCCCCGGCGCCCAGCGAGGA152700.10878931456676098No Hit
CATTACCAGCAGCAGCAGCACATAGCTACACTGATTAAAAGATTAAAAAA151140.10767791096018503No Hit
TACCAGCAGCAGCAGCAGAGCGAGCTGCAGCCCCGGCGCCCAGCGAGGAT144850.10319667462341407No Hit
TACCAGCAGCAGCAGCACATAGCTACACTGATTAAAAGATTAAAAAAACT143620.10232037562592149No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)