FastQCFastQC Report
Mon 22 May 2023
EGAF00008003441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6296416
Sequences flagged as poor quality0
Sequence length130
%GC55

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGTCCACTATACTG63104210.022241224213902No Hit
TGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGTCCACTATACTGA5732469.104322204886081No Hit
TGCCTCCCTGAACCACTCCACTGCCTTTAACCCTCAGCCTCAGAGTCAGA5166578.205572821109659No Hit
ATGCCTCCCTGAACCACTCCACTGCCTTTAACCCTCAGCCTCAGAGTCAG4863907.724870783633102No Hit
CATTGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGTCCACTATAC4838547.684593902308869No Hit
TGTCTTCCGGGAGCCCATCATCTGCAAAAACATCCCACGCCTAGTCCCTG3954236.280128250738199No Hit
CATTGCCTCCCTGAACCACTCCACTGCCTTTAACCCTCAGCCTCAGAGTC3878666.160107591366263No Hit
TTTGCCTCCCTGAACCACTCCACTGCCTTTAACCCTCAGCCTCAGAGTCA3809046.049536752336567No Hit
ATGTCTTCCGGGAGCCCATCATCTGCAAAAACATCCCACGCCTAGTCCCT3792156.022711968205405No Hit
TTTGTCTTCCGGGAGCCCATCATCTGCAAAAACATCCCACGCCTAGTCCC2991294.750782032191012No Hit
CATTGTCTTCCGGGAGCCCATCATCTGCAAAAACATCCCACGCCTAGTCC2731944.338881039626353No Hit
TTTGGTGCACTGAAATGGAAAGGGTATTTGGTTTCCCAGTCCACTATACT2512693.9906670715530868No Hit
ATGTCTTCCGGGAGCCCATCATCTGCAAAAAAAAAAAAAAAAAAAAAAAA468990.7448523096313839No Hit
TGTCTTCCGGGAGCCCATCATCTGCAAAAAAAAAAAAAAAAAAAAAAAAA462340.7342907457194696No Hit
TTTGTCTTCCGGGAGCCCATCATCTGCAAAAAAAAAAAAAAAAAAAAAAA381720.6062496505948781No Hit
CATTGTCTTCCGGGAGCCCATCATCTGCAAAAAAAAAAAAAAAAAAAAAA368550.5853329894339891No Hit
TGCCTCCCTGAACCACTCCACTGCCTTTTAACCCTCAGCCTCAGAGTCAG288180.45768894558428164No Hit
ATGCCTCCCTGAACCACTCCACTGCCTTTTAACCCTCAGCCTCAGAGTCA274380.4357717152106849No Hit
CATTGCCTCCCTGAACCACTCCCCCATTCCTTTCCCTCCTCCCTCGTCAT231950.36838417283737285No Hit
CATTGCCTCCCTGAACCACTCCACTGCCTTTTAACCCTCAGCCTCAGAGT213670.339351783617855No Hit
TTTGCCTCCCTGAACCACTCCACTGCCTTTTAACCCTCAGCCTCAGAGTC210200.33384071192246506No Hit
TGCCTCCCTGAACCACTCCCCCATTCCTTTCCCTCCTCCCTCGTCATAGA201680.32030920447441846No Hit
ATGCCTCCCTGAACCACTCCCCCATTCCTTTCCCTCCTCCCTCGTCATAG191800.3046177380910029No Hit
TTTGCCTCCCTGAACCACTCCCCCATTCCTTTCCCTCCTCCCTCGTCATA177930.28258933336043873No Hit
AGTGCTTGTCACCCACGGGAAAGTGGTGAAGATATGTGACTTTGGATTGG140250.22274576520992262No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG128820.2045925809222262No Hit
TTGTGCTTGTCACCCACGGGAAAGTGGTGAAGATATGTGACTTTGGATTG104830.16649154058435783No Hit
TGCCTCCCTGAACCACTCCACTGCCTTTAACCCTCCGTTTGCCTTAGCGT98020.15567586385651774No Hit
ATGCCTCCCTGAACCACTCCACTGCCTTTAACCCTCCGTTTGCCTTAGCG94890.15070478189497008No Hit
GTGCTTGTCACCCACGGGAAAGTGGTGAAGATATGTGACTTTGGATTGGC82440.130931628405747No Hit
CATTGCCTCCCTGAACCACTCCACTGCCTTTAACCCTCCGTTTGCCTTAG74990.11909950041420389No Hit
TTTGCCTCCCTGAACCACTCCACTGCCTTTAACCCTCCGTTTGCCTTAGC71950.1142713569116145No Hit
CATGTGCTTGTCACCCACGGGAAAGTGGTGAAGATATGTGACTTTGGATT68420.10866499291025244No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)