FastQCFastQC Report
Wed 31 May 2023
EGAF00008003444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27948380
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTTGAGTGTACCTCTAACTTCTAAC1529700.5473304713904705No Hit
CTGCATCGTACCATACTACCGCGGGGTT955520.34188743676735467No Hit
GCCATGGAGCGCTTCGAATCGCCACGTA946660.33871730669183686No Hit
GGTAGAGGTACTCAACCCCAACCGTTGC933650.3340622962762063No Hit
ACATCCCGTAGCGTTTCTCGTAAGGGCC931980.33346476611524534No Hit
ACATCCCTCGCCGTGAGGTATATACTAA928770.33231622011723044No Hit
ACCATTTCAGCGTAGATACAGGCAATGA905790.32409391886041333No Hit
GCGAGAAGTTGCGGAATTTAGGCGCTTG884810.3165872225867832No Hit
GCACATACACTCACTCTGTGTTTTAGGT769220.27522883258349856No Hit
GTCTCACAGCAAGTGCACGTCCAAATGC733020.26227638238781636No Hit
AATCGACCAAATTAGGTTGCGGATGCTA709210.25375710506297683No Hit
TTGTTCACAGATAAACCTCCCAGCATTA649110.23225317531821166No Hit
ATTCTACTCCTACTGCATCCATTTAGCG630990.22576979417053866No Hit
CCCGAAGCAGGTGGATCCTCTAGAATTG624540.22346196809976104No Hit
AGACCCGAGAACGCGTGTATTCTCAGTC621780.2224744332229632No Hit
TACCGAACAAAGACGCAGTTTTTAACGG614990.2200449543050438No Hit
TCCTTCTCAACAGCCCACCTTGACCGCA607840.21748666649015078No Hit
CATGGTATCCACGTCTCAATAGTTGTAA597470.2137762546523269No Hit
CTCTCAGTCCATCTATGACATCGCGCTA596510.2134327642603972No Hit
ACATTTCCAAGCGCTCAGTAACCTGGGC585560.20951482697744916No Hit
CTTCAATGTCTCGCGACTATCAAGAGTC573470.20518899485408457No Hit
CCTCATGGTATCTCTTGTCGACTACATA558620.19987562785392213No Hit
GTGGAGACAGAACTTCAGTAACTAAATC548960.1964192557851296No Hit
ACGTCCTAGACCGCCTGGCTTATGGAAT545440.19515979101472072No Hit
CTGGACGAGCTGAAGCGAGGGTTATTAG494440.17691186394345576No Hit
GTGTTAGTCATGACACTACGCCTTTCAT491970.17602809178922No Hit
CTCCCTCCAAGCTACTATTATTGAACTT491620.17590286091716228No Hit
ACACAGTGTCAACCTACAATAGACGCTA482700.17271126269214887No Hit
TAGAGTCTCGCCACTTTCAGGCTTCCCG480920.17207437425711258No Hit
TTCAGGACACTTCAAGTTTTCAAGATAT480910.17207079623219665No Hit
TTCGCTGGTACAGTCTTCTTTACACTGT465930.16671091490812706No Hit
ACTATCTTCTCGACCTCAGCTCCTTGAT464080.1660489802986792No Hit
CAGTTCCAGTGAGTGCGAGATGATTACT453510.16226700796253665No Hit
GTTGTAGTCACCCTTGCCGCAGTCAATT429190.15356525136698443No Hit
TATCTGTTCCCATAAGAGGTGAATGCTA421210.15070998748406883No Hit
TATGTTCCAAATCAGACATTTTATATAC418400.14970456248269132No Hit
TGTCAGATCTTCGATTAAAGTGTTTCTC411920.14738600233716587No Hit
ATGAGTCGTAGGTGCAACAATACGCGGG410020.14670617760313837No Hit
ATTCACTGTATAATGGCACCTATTCACG399920.1430923724380447No Hit
TGACTCCTCGAAGCCCAAGTGCCCAACA385190.13782194173687348No Hit
ACTATCTTCCGTTTCGTATTGCACGCGT381830.13661972536511954No Hit
TTCGATTGTTGCGGCTTTGCTTCAGTGC363210.12995744297164988No Hit
TGTGGCGTCTCCGTGTAAATGGCTAGAT362370.12965688887871138No Hit
AATGGCTCAGAGGAAATGTCGCCGAAGC356090.12740988923150465No Hit
ACTTATCAGTCATGCTCTAGTGTCTGTG355210.12709502303890244No Hit
TGTTGAGTCTCAGTCCCTTATCACACAT345240.12352773219771593No Hit
GCTGAATTCTCTCGCACTTAAACGTAAA341250.12210010025625814No Hit
ATTCGTTAGCGTCTGCATTCGTCGGGCA328940.11769555158474303No Hit
GAGACTTCAAATCCCAAACTACGCGTTT326540.11683682560491879No Hit
TGCGGCAGCTTGTTTTCTGTTCGTATTT324420.11607828432274071No Hit
TACCCACAGCTAATCCGTCAAAGTCCAG319260.11423202346611862No Hit
GAGGCCTCAAGGGCATGCAGCACCACGT317350.11354862070717514No Hit
TACTTGTTCTCGCTTGTTTGTTACCATG316390.11320513031524546No Hit
GTAGGTTGTTTGTTGGCTCGACTGGCTG304730.10903315326326606No Hit
CGGAGAATCTCCATATCACATTTCGCTA295000.10555173502006197No Hit
CTCAGGGTCCGGCTTTTCCTCCCCTTAT284120.10165884391152546No Hit
TACGGTATCTATGTGGAGTCCCGGTTTG282590.10111140609938751No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)