FastQCFastQC Report
Wed 31 May 2023
EGAF00008003447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5311198
Sequences flagged as poor quality0
Sequence length130
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGGAGAGGCTAATGCTGAGCTCTGTCGGTATCTTTCCAAGGTCCTAGA122463123.05752864043103No Hit
AGGGAGAGGCTAATGCTGAGCTCTGTCGGTATCTTTCCAAGGTCCTAGAA120346022.658918006822564No Hit
CATAGGGAGAGGCTAATGCTGAGCTCTGTCGGTATCTTTCCAAGGTCCTA96021718.07910381047741No Hit
TTAGGGAGAGGCTAATGCTGAGCTCTGTCGGTATCTTTCCAAGGTCCTAG4223137.9513699169189325No Hit
ACTGGGAGCATGGCAGTGGAGTAACAGTAAGGGTTGGAGTCACAGTAGCA1383422.6047230775429573No Hit
CATCTGGGAGCATGGCAGTGGAGTAACAGTAAGGGTTGGAGTCACAGTAG850711.6017290261067276No Hit
TTCTGGGAGCATGGCAGTGGAGTAACAGTAAGGGTTGGAGTCACAGTAGC793161.4933730582064537No Hit
CTGGGAGCATGGCAGTGGAGTAACAGTAAGGGTTGGAGTCACAGTAGCAC724351.3638166003225638No Hit
GGCCCCACCACCAGTTTTGAGTCTGCCGCCTGCCCCGGTTGGTTCCTCTG331680.6244918754676441No Hit
AGGCCCCACCACCAGTTTTGAGTCTGCCGCCTGCCCCGGTTGGTTCCTCT324260.6105213927253325No Hit
CATGGCCCCACCACCAGTTTTGAGTCTGCCGCCTGCCCCGGTTGGTTCCT263330.49580151220120205No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG258400.48651923727942353No Hit
TTGGCCCCACCACCAGTTTTGAGTCTGCCGCCTGCCCCGGTTGGTTCCTC258120.4859920492514118No Hit
AACACTTCGAGCACTGTCCAGTATTCTACCGTGGTACACAGTGGCTACAG244020.4594443664122482No Hit
ACACTTCGAGCACTGTCCAGTATTCTACCGTGGTACACAGTGGCTACAGA231650.43615395246044303No Hit
TAGGGAGAGGCTAATGCTGAGCTCTGTCGGTATCTTTCCAAGGTCCTAGA154720.29130904176421213No Hit
CATACACTTCGAGCACTGTCCAGTATTCTACCGTGGTACACAGTGGCTAC149200.2809159063548375No Hit
TTACACTTCGAGCACTGTCCAGTATTCTACCGTGGTACACAGTGGCTACA139040.26178651219555366No Hit
CATAGGGAGAGCTAATGCTGAGCTCTGTCGGTATCTTTCCAAGGTCCTAG127270.2396257868752022No Hit
AGGGAGAGCTAATGCTGAGCTCTGTCGGTATCTTTCCAAGGTCCTAGAAC76190.14345162805077122No Hit
ACTGGGAGCATGGCAGTGGAGGTTGCAGTGAGCCGAGATCACGCCACTGC74920.14106045378086074No Hit
AAGGGAGAGCTAATGCTGAGCTCTGTCGGTATCTTTCCAAGGTCCTAGAA67020.1261862201333861No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)