FastQCFastQC Report
Sun 21 May 2023
EGAF00008003463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1447807
Sequences flagged as poor quality0
Sequence length130
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGCAGGGCAAAGACCAGCATTTTCCTGTCTTCATGAATGAGAAAGAGG24917517.21051217461996No Hit
AAGCAGGGCAAAGACCAGCATTTTCCTGTCTTCATGAATGAGAAAGAGGA24804617.13253216761626No Hit
CATAAGCAGGGCAAAGACCAGCATTTTCCTGTCTTCATGAATGAGAAAGA20385314.080122557771857No Hit
TTAAGCAGGGCAAAGACCAGCATTTTCCTGTCTTCATGAATGAGAAAGAG19851813.71163421643907No Hit
ACTCGCCCTCCTACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCC192861.3320836271685383No Hit
CTCGCCCTCCTACCACCTGTCCAAAAAGGCCTTCGATACGGGATAATCCT180491.2466440623646662No Hit
ACTCGCCCTCCTACCACCTGTCCTTCGAGCAGGAGTTAACACCGTCACCA139060.9604871367523434No Hit
CTCGCCCTCCTACCACCTGTCCTTCGAGCAGGAGTTAACACCGTCACCAC131440.9078558122733209No Hit
CATCTCGCCCTCCTACCACCTGTCCAAAAAGGCCTTCGATACGGGATAAT118470.8182720486915729No Hit
CATCTCGCCCTCCTACCACCTGTCCTTCGAGCAGGAGTTAACACCGTCAC85180.5883380864990984No Hit
ACTCGCCCTCCTACCACCTGTCCCTCCTGGGCTGCTGGATTGTCTCGTTT60170.4155940674413095No Hit
CTCGCCCTCCTACCACCTGTCCCTCCTGGGCTGCTGGATTGTCTCGTTTT54950.3795395380737902No Hit
CCCTTGCCGTCCCAAGCAATGGATGATTTGATGCTGTCCCCGGACGATAT51270.3541217855694855No Hit
ACCCTTGCCGTCCCAAGCAATGGATGATTTGATGCTGTCCCCGGACGATA43730.3020430209275131No Hit
ACTCGCCCTCCTACCACCTGTAGAGCCAAAAAAAAAAAAAAAAAAAAAAA35790.24720145710029032No Hit
CATCTCGCCCTCCTACCACCTGTCCCTCCTGGGCTGCTGGATTGTCTCGT35700.24657982728360892No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33150.22896698247763686No Hit
CTCGCCCTCCTACCACCTGTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAA31510.21763950581810973No Hit
CATCCCTTGCCGTCCCAAGCAATGGATGATTTGATGCTGTCCCCGGACGA27720.19146198353786104No Hit
TTCCCTTGCCGTCCCAAGCAATGGATGATTTGATGCTGTCCCCGGACGAT27090.1871105748210915No Hit
ACTCGCCCTCCTACCACCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAA26350.18199939632837803No Hit
ACTCGCCCTCCTACCACCTTCAGAAGTCGACCTGTGGCAAATGTGGCTAC23810.1644556215020372No Hit
CTCGCCCTCCTACCACCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAAAAA23070.15934444300932377No Hit
CATCTCGCCCTCCTACCACCTGTAGAGCCAAAAAAAAAAAAAAAAAAAAA22920.1583083933148548No Hit
CTCGCCCTCCTACCACCTTCAGAAGTCGACCTGTGGCAAATGTGGCTACC20760.143389277714502No Hit
CATCTCGCCCTCCTACCACCTCTAGAGCCAAAAAAAAAAAAAAAAAAAAA19390.13392669050501899No Hit
AAGCAGGGCAAAGCCCAGCATTTTCCTGTCTTCATGAATGAGAAAGAGGA19140.1321999410142374No Hit
AAAGCAGGGCAAAGCCCAGCATTTTCCTGTCTTCATGAATGAGAAAGAGG18250.126052712827055No Hit
ACTCGCCCTCCTACCACCTGTCCAGAAGCTGCCTTTGAAGTCAGTTCTGG17560.12128688423249784No Hit
CTCGCCCTCCTACCACCTGTCCAGAAGCTGCCTTTGAAGTCAGTTCTGGG16850.11638291567867816No Hit
CATCTCGCCCTCCTACCACCTTCAGAAGTCGACCTGTGGCAAATGTGGCT15890.10975219763407693No Hit
ACTCGCCCTCCTACCACCTGCCCATGGCCTGGGACTCTCTGCGATGTACA15180.10484822908025725No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)