FastQCFastQC Report
Wed 31 May 2023
EGAF00008003474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83597
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT2357728.20316518535354No Hit
GCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA1654219.787791427922055No Hit
TGTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG73238.759883727884972No Hit
GCCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACTA63097.546921540246659No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAA55366.622247209828104No Hit
TGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTG34004.067131595631422No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGACGGA30083.5982152469586226No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGTTGGA19612.345777958539182No Hit
ACACATATGGCCTAGACTACGTACATAACCCAAACCTACTCCAATGCTAA17512.0945727717501823No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG7340.8780219385863128No Hit
GATCCTCGTGGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT5960.7129442444106846No Hit
TCAGCGTGCTCTTGCTAAAACTGGAGCTGAATCTATCAGTTTGCTTGAGT4150.49642929770207067No Hit
TAGTGAGCATGTCCCCGCTGCAGCCCACCTCCAGTGTCATGCCCTCCAGG4100.4904482218261421No Hit
TAGTGAGCATGTCCCCGCTGCAGCCCACCTCCAGTGTCGTGCCCTCCAGG4060.48566336112539926No Hit
GATCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT3880.46413148797205644No Hit
GCCCTACGCGCAGAGGAGCGCCGCCGCGGCGTACAGCCACCAGCCCATCA3810.45575798174575644No Hit
GCCCTACGCGCAGAGGAGCGCCGCCGCGGCGTACGGCCACCAGCCCATCA3760.4497769058698278No Hit
GGATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT3710.4437958299938993No Hit
TTTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTG3580.428245032716485No Hit
AGGGCCTGCCTTAAGTGTGAAAGTAATGACTGATGAAAGTGGAAAATCCA3170.3792002105338708No Hit
TCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA2400.287091642044571No Hit
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCGAGT2380.28469921169419954No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATTCTAA2080.24881275643862819No Hit
ACACCCAGCCTTGGCTAGCTGGAACACCTGTCTCTTATACACATCTCCGA2080.24881275643862819No Hit
GGCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA1870.2236922377597282No Hit
TCCCACAGGACAACCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC1820.21771116188379966No Hit
TCCCACAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC1790.2141225163582425No Hit
GATCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACT1570.18780578250415686No Hit
GATCCTCGTGGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACT1410.16866633970118544No Hit
AATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA1290.1543117575989569No Hit
CCGTTTTCTTGTAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGACGGA1220.1459382513726569No Hit
TCCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACTA1060.1267988085696855No Hit
TCAGCGTGCTCTTGCTAAAACTAATCGTCCCAACAATTATATTACTACCA970.11603287199301411No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)