FastQCFastQC Report
Wed 31 May 2023
EGAF00008003476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80049
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT3598944.958712788417095No Hit
GCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA1756221.93906232432635No Hit
TGTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG1088613.59917050806381No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAA42405.296755737111019No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG10151.2679733663131332No Hit
GGATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT6410.8007595347849443No Hit
GATCCTCGTGGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT6200.7745256030681208No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGACGGA5000.6246174218291297No Hit
GATCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT3960.4946969980886707No Hit
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCGAGT3070.3835150970030856No Hit
TCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA2690.33604417294407174No Hit
GCCCTACGCGCAGAGGAGCGCCGCCGCGGCGTACGGCCACCAGCCCATCA2660.33229646841309696No Hit
TCCCACAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC2420.30231483216529875No Hit
GGCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA2360.2948194231033492No Hit
TCCCACAGGACAACCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC2270.28357630951042484No Hit
AATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA1850.23110844607677794No Hit
ACACCCAGCCTTGGCTAGCTGGAACACCTGTCTCTTATACACATCTCCGA1710.21361915826556233No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGTTTCGTTTG1390.17364364326849804No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATTCTAA1340.16739746905020675No Hit
GATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA1090.13616659795875027No Hit
ACACATATGGCCTAGACCTCAACTACCTAACCAACAAACTTAAAATAAAA940.11742807530387638No Hit
GAATGACTGAGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT830.10368649202363552No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)