FastQCFastQC Report
Wed 31 May 2023
EGAF00008003492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89280
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT3516439.38620071684588No Hit
GCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA2291225.663082437275985No Hit
TGTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG1223113.699596774193548No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAA53385.978942652329748No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG13281.4874551971326164No Hit
GATCCTCGTGGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT8860.992383512544803No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGACGGA7140.799731182795699No Hit
GATCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT5610.6283602150537635No Hit
GGATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT5130.5745967741935484No Hit
GGCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA4030.45138888888888884No Hit
GCCCTACGCGCAGAGGAGCGCCGCCGCGGCGTACGGCCACCAGCCCATCA3480.3897849462365591No Hit
TCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA3430.3841845878136201No Hit
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCGAGT2830.31698028673835127No Hit
TCCCACAGGACAACCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC2660.2979390681003584No Hit
TCCCACAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC2430.2721774193548387No Hit
AATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA1720.19265232974910396No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATTCTAA1630.18257168458781362No Hit
AGGGCCTGCCTTAAGTGTGAAAGTAATGACTGATGAAAGTGGAAAATCCA1470.1646505376344086No Hit
TAGTGAGCATGTCCCCGCTGCAGCCCACCTCCAGTGTCGTGCCCTCCAGG1440.16129032258064516No Hit
ACACCCAGCCTTGGCTAGCTGGAACACCTGTCTCTTATACACATCTCCGA1430.16017025089605735No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGTTTCGTTTG1290.14448924731182794No Hit
GATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA1090.12208781362007168No Hit
AGGGCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAAC950.1064068100358423No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)