FastQCFastQC Report
Sun 21 May 2023
EGAF00008003502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87454
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT4101346.89665424108674No Hit
GCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA81529.321471859491847No Hit
TGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTG67967.770942438310426No Hit
GCCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACTA67737.744642897980652No Hit
TGTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG66887.647448944588012No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGACGGA20072.294920758341528No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGTTGGA17532.004482356438814No Hit
GCCCTACGCGCAGAGGAGCGCCGCCGCGGCGTACAGCCACCAGCCCATCA14381.6442929997484392No Hit
GCCCTACGCGCAGAGGAGCGCCGCCGCGGCGTACGGCCACCAGCCCATCA10841.239508770324971No Hit
TTTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTG7490.8564502481304458No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG6920.7912731264436161No Hit
GGATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT5350.6117501772360326No Hit
TAGTGAGCATGTCCCCGCTGCAGCCCACCTCCAGTGTCGTGCCCTCCAGG3910.4470921856061473No Hit
ACACCCAGCCTTGGCTAGCTGGAACACCTGTCTCTTATACACATCTCCGA3850.440231435954902No Hit
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCGAGT2940.3361767329110161No Hit
GATCCTCGTGGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT2110.24126969606879045No Hit
AATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA2090.23898277951837538No Hit
TAGTGAGCATGTCCCCGCTGCAGCCCACCTCCAGTGTCATGCCCTCCAGG2030.23212202986713013No Hit
ACACCCAGCCTCGTCCAGCTGGAACACCTGTCTCTTATACACATCTCCGA1480.16923182473071557No Hit
ACACCCAGCCTTGTCCAGCTGGAACACCTGTCTCTTATACACATCTCCGA1440.16465799162988543No Hit
GATCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT1340.15322340887781005No Hit
GGCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA1280.14636265922656483No Hit
GATCCTCGTGGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACT1250.14293228440094222No Hit
GATCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACT1250.14293228440094222No Hit
TCCCACAGGACAACCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC1110.1269238685480367No Hit
GAATGACTGAGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT1070.12235003544720655No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAA1050.12006311889679146No Hit
TCCCACAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC1000.11434582752075377No Hit
TCCCACAGGACAACCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTC1000.11434582752075377No Hit
GATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA990.11320236924554623No Hit
TCCCACAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTC960.10977199441992362No Hit
GGCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACTA900.10291124476867838No Hit
TTTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGTTTCGTTTG880.10062432821826331No Hit
TCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA880.10062432821826331No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)