FastQCFastQC Report
Sun 21 May 2023
EGAF00008003512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80996
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT2621932.37073435725221No Hit
GCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA2249927.777914958763393No Hit
TGTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG1152114.224159217739146No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAA60087.417650254333548No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGTTGGA17582.170477554447133No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG10021.2370981283026323No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGACGGA9351.1543779939750112No Hit
GCCCTACGCGCAGAGGAGCGCCGCCGCGGCGTACGGCCACCAGCCCATCA9031.1148698701170427No Hit
GATCCTCGTGGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT6000.7407773223369055No Hit
ACACCCAGCCTTGGCTAGCTGGAACACCTGTCTCTTATACACATCTCCGA4150.5123709812830263No Hit
GGATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT4110.5074324658007803No Hit
TAGTGAGCATGTCCCCGCTGCAGCCCACCTCCAGTGTCGTGCCCTCCAGG2510.3098918465109388No Hit
AGGGCCTGCCTTAAGTGTGAAAGTAATGACTGATGAAAGTGGAAAATCCA2400.2963109289347622No Hit
TCAGCGTGCTCTTGCTAAAACTGGAGCTGAATCTATCAGTTTGCTTGAGT2240.27655686700577803No Hit
GATCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT2230.27532223813521656No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATTCTAA2230.27532223813521656No Hit
GGCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA2030.25062966072398635No Hit
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCGAGT1720.2123561657365796No Hit
ACACCCAGCCTCGTCCAGCTGGAACACCTGTCTCTTATACACATCTCCGA1580.19507136154871846No Hit
TCTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG1550.19136747493703393No Hit
ACACCCAGCCTTGTCCAGCTGGAACACCTGTCTCTTATACACATCTCCGA1420.1753172996197343No Hit
TCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA1330.16420563978468072No Hit
AATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA1250.15432860882018867No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGTTTCGTTTG1220.15062472220850412No Hit
TCCCACAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC1110.13704380463232751No Hit
TCCCACAGGACAACCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC1040.12840140253839696No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)