FastQCFastQC Report
Sun 21 May 2023
EGAF00008003521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68468
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC1407820.56143015715371No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT1382620.19337500730268No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG761911.127826137757785No Hit
GGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT32884.802243383770521No Hit
GTGTTCCAGCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACT14862.170356955073903No Hit
GGCTGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT8041.1742711923818425No Hit
GGCTGTGGGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT7521.0983233043173453No Hit
GGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC7461.0895600864637496No Hit
CAACGCGCTCACTTCCTTCGTGGACGCCAGCATGGTGTACGGCAGCGAGG5580.8149792603844133No Hit
TGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC3570.5214114622889525No Hit
TGCGGCGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT3540.5170298533621546No Hit
TGGGTTTTGCCTGCATACGACAAACAGACCTAAAATCGCTCATTGCATAC2710.3958053397207455No Hit
TCCTTGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAA2440.35637085937956414No Hit
TGCGGTGGGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT2410.35198925045276624No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGAATGTTGGAGAAATAAAAT2020.2950283344043933No Hit
AGCTCCATCTGCATACGACAAACAGACCTAAAATCGCTCATTGCATACTC1880.2745808260793363No Hit
GGCTGTGTGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT1820.26581760822574046No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGTAATAAAAT1750.255593854063212No Hit
AGCTCCATCTGCCTACGAGAAACAGACCTAAAATCGCTCATTGCATACTC1490.21761991003096337No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGCAATAAAAT1450.2117777647952328No Hit
GTTTGGACCCCAGACCTGCTGAACAACCACATCTTGAAGTCAGCTATGTG1410.20593561955950224No Hit
GGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT1400.2044750832505696No Hit
CCGACCTGGCTTCCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG1360.19863293801483906No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATAGCATACTC1320.1927907927791085No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATCGCATACTC1220.17818542968978207No Hit
TGCGGTGTGTGATGCTAGGGTAGAGTCCGAGTATGTTGGAGAAATAAAAT1170.1708827481451189No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGGTGGAGAAATAAAAT1140.16650113921832096No Hit
AGCTCCATCTGCCTACGACAAACAAACCTAAAATCGCTCATTGCATACTC1100.1606589939825904No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGGAATAAAAT1100.1606589939825904No Hit
GGCTGTGGGTGGTTGTGTTGATTCAAATTATGAGTTTTTTGGAAAGTCAT1090.15919845767365776No Hit
TGCGGTGTGTGATGCTAGCGTAGAATCCGAGTATGGTGGAGAAATAAAAT1080.15773792136472511No Hit
GGCTGTGGGTGGTTGTGTTGATTAAAATTATGTGTTTTTTGGAAAGTCAT1080.15773792136472511No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGGGTATGTTGGAGAAATAAAAT1050.1533563124379272No Hit
AGCTCCATCTGCCTACGACAAACAGACCGAGAATCGCTCATTGCATACTC1000.146053630893264No Hit
CCGACCTGGATTCCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG970.14167202196646608No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAATTTAATCG970.14167202196646608No Hit
TGCGGTGTGAGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT950.1387509493486008No Hit
AGCTCCAGCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC950.1387509493486008No Hit
GTGTTCCAGCTGGACGAGGCCGACGAGGCGGCGGCTGCGGCAGCAGCGGC940.13729041303966816No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAGAATCGCTCATTGCATACTC930.13582987673073552No Hit
GGCTGCGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT920.13436934042180287No Hit
GTGTTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACT910.13290880411287023No Hit
TGCGGTGTGTGCTGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT900.1314482678039376No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGAACACGTAGGACTTTAATCG900.1314482678039376No Hit
GTTTTCCAGCTGCCTACGACAAACAGACCTAAACTCGCTCATTGCATACT870.1270666588771397No Hit
TGCGGTGTGTGATGCTAGCGTAGAATCCGAGTATGTTGGAGAAATAAAAT860.12560612256820705No Hit
AGCTCTATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC860.12560612256820705No Hit
CCGACCTGGCTTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG820.11976397733247648No Hit
TCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG810.11830344102354384No Hit
TGCGGTGTGTGAAGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT790.11538236840567856No Hit
AGCTCCATCTGTCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC790.11538236840567856No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGATTTTAATCG750.109540223169948No Hit
TGCGGTGTGTGATGCTAGGGTAGCATCCGAGTATGTTGGAGAAATAAAAT740.10807968686101536No Hit
TGCGGTGTGTGATGATAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT740.10807968686101536No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAGTTTAATCG740.10807968686101536No Hit
GTGTTCCAGCTAGCCAAGGCTGGGTGTCTGTCTCTTATACACATCTGACG720.10515861424315008No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATGGCATACTC700.1022375416252848No Hit
ACTCGCCATTCTCATCCAAACCCCCTGAAGCTTCACCGGCGCAGTCATTC700.1022375416252848No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)