FastQCFastQC Report
Wed 31 May 2023
EGAF00008003536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87478
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT2482128.37399117492398No Hit
GCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA2250125.721895790941723No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAA1317115.056357026909623No Hit
TGTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG1122712.834083998262422No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGACGGA17662.018793296600288No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGTTGGA10871.242598138960653No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG9721.1111365143236014No Hit
GATCCTCGTGGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT6930.7921991815084936No Hit
GCCCTACGCGCAGAGGAGCGCCGCCGCGGCGTACGGCCACCAGCCCATCA6110.6984613274194654No Hit
TCAGCGTGCTCTTGCTAAAACTGGAGCTGAATCTATCAGTTTGCTTGAGT5430.6207274971992958No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATTCTAA4740.5418505224170649No Hit
GGATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT4160.4755481378175084No Hit
TAGTGAGCATGTCCCCGCTGCAGCCCACCTCCAGTGTCGTGCCCTCCAGG3550.40581631953176794No Hit
TAGTGAGCATGTCCCCGCTGCAGCCCACCTCCAGTGTCATGCCCTCCAGG2940.33608450124602757No Hit
GATCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT2820.32236676650129176No Hit
GGCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA2760.31550789912892385No Hit
ACACCCAGCCTTGGCTAGCTGGAACACCTGTCTCTTATACACATCTCCGA2160.24691922540524472No Hit
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCGAGT1840.21033859941928254No Hit
AATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA1390.15889709412652323No Hit
TCCCACAGGACAACCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC1300.14860879306797137No Hit
TCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA1270.14517935938178742No Hit
TCCCACAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC1250.1428930702576648No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGTTTCGTTTG1250.1428930702576648No Hit
AGACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAA1100.125745901826745No Hit
TCAGCGTGCTCTTGCTAAAACTAATCGTCCCAACAATTATATTACTACCA1060.12117332357849975No Hit
ACACATATGGCCTAGACTACGTACATACCCTAAACCTACTCCAATTCTAA990.1131713116440705No Hit
TCTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG970.11088502251994786No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)