FastQCFastQC Report
Wed 31 May 2023
EGAF00008003539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65979
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC1454622.04640870580033No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT1171417.754133891086557No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG732111.095954773488534No Hit
CAACGCGCTCACTTCCTTCGTGGACGCCAGCATGGTGTACGGCAGCGAGG17112.5932493672229042No Hit
GTGTTCCAGCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACT13992.1203716333985057No Hit
GTGTTCCAGCTGGACGAGGCCGACGAGGCGGCGGCTGCGGCAGCAGCGGC7901.1973506721835736No Hit
GGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC7901.1973506721835736No Hit
GGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT7131.0806468724897316No Hit
GGCTGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT7061.0700374361539278No Hit
GGCTGTGGGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT5560.8426923718152745No Hit
TGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC3880.5880658997559829No Hit
TGCGGCGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT2930.4440806923415026No Hit
TGGGTTTTGCCTGCATACGACAAACAGACCTAAAATCGCTCATTGCATAC2440.3698146379908759No Hit
TGCGGTGGGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT2320.3516270328437836No Hit
AGCTCCATCTGCATACGACAAACAGACCTAAAATCGCTCATTGCATACTC2100.3182830900741145No Hit
TCCTTGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAA1910.2894860485912184No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGAATGTTGGAGAAATAAAAT1750.2652359083950954No Hit
CCGACCTGGCTTCCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG1720.2606890071083223No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATAGCATACTC1600.2425014019612301No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATCGCATACTC1580.23947013443671472No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGTAATAAAAT1460.22128252928962244No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGCAATAAAAT1460.22128252928962244No Hit
AGCTCCATCTGCCTACGAGAAACAGACCTAAAATCGCTCATTGCATACTC1380.20915745919156095No Hit
AGCTCCATCTGCCTACGACAAACAAACCTAAAATCGCTCATTGCATACTC1340.2030949241425302No Hit
AGCTCCAGCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC1300.19703238909349946No Hit
GTGTTCCAGCTAGCCAAGGCTGGGTGTCTGTCTCTTATACACATCTGACG1140.17278224889737645No Hit
GGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT1110.16823534761060335No Hit
CCGACCTGGATTCCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG1060.16065717879931493No Hit
TCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG1050.15914154503705724No Hit
AGCTCCATCTGCCTACGACAAACAGACCGAGAATCGCTCATTGCATACTC1050.15914154503705724No Hit
TGCGGTGTGTGATGCTAGGGTAGAGTCCGAGTATGTTGGAGAAATAAAAT1010.15307900998802648No Hit
AGCTCTATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC1000.15156337622576882No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAATTTAATCG1000.15156337622576882No Hit
CCGACCTGGCTTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG980.14853210870125344No Hit
GTGTTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACT950.14398520741448037No Hit
ACTCGCCATTCTCATCCAAACCCCCTGAAGCTTCACCGGCGCAGTCATTC900.13640703860319192No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAGAATCGCTCATTGCATACTC890.13489140484093423No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATGGCATACTC880.13337577107867654No Hit
TGCGGTGTGTGATGCTAGCGTAGAATCCGAGTATGGTGGAGAAATAAAAT880.13337577107867654No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGGTGGAGAAATAAAAT880.13337577107867654No Hit
AGCTCCATCTGCCTACGACAAACAGCCCTAAAATCGCTCATTGCATACTC860.13034450355416116No Hit
AGCTCCATCTGCCTACGACAAACAGTCCTAAAATCGCTCATTGCATACTC860.13034450355416116No Hit
GTTTGGACCCCAGACCTGCTGAACAACCACATCTTGAAGTCAGCTATGTG820.12428196850513042No Hit
TGCGGTGTGAGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT800.12125070098061505No Hit
AGGTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC790.11973506721835736No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGATTTTAATCG780.11821943345609967No Hit
AGCTCCATCTGCCTACGACAAACAGACCTGAAATCGCTCATTGCATACTC760.1151881659315843No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAGTTTAATCG740.11215689840706891No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGGAATAAAAT730.11064126464481122No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAAATCACGTAGGACTTTAATCG720.10912563088255353No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGGGTATGTTGGAGAAATAAAAT720.10912563088255353No Hit
TGCGGTGTGTGATGCTAGGGTAGCATCCGAGTATGTTGGAGAAATAAAAT710.10760999712029586No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAACTCGCTCATTGCATACTC700.10609436335803817No Hit
GTTTTCCAGCTGCCTACGACAAACAGACCTAAACTCGCTCATTGCATACT700.10609436335803817No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGAACACGTAGGACTTTAATCG670.1015474620712651No Hit
GTTTTCCAGCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACT660.10003182830900742No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTTCATACTC660.10003182830900742No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)