FastQCFastQC Report
Sun 21 May 2023
EGAF00008003540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48475
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT1786036.843733883445076No Hit
GCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA816716.84785972150593No Hit
GCCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACTA518010.68592057761733No Hit
TGTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG32856.776689014956163No Hit
TGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTG25155.1882413615265595No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAA24134.977823620422899No Hit
GCCCTACGCGCAGAGGAGCGCCGCCGCGGCGTACGGCCACCAGCCCATCA4770.9840123775141825No Hit
GGATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT4680.9654461062403301No Hit
GCCCTACGCGCAGAGGAGCGCCGCCGCGGCGTACAGCCACCAGCCCATCA4450.9179989685404849No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGTTGGA4020.8292934502320783No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGACGGA3810.7859721505930892No Hit
GATCCTCGTGGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT2840.5858690046415679No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG2580.5322331098504384No Hit
TTTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTG1880.38782877772047447No Hit
GGCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA1570.32387828777720473No Hit
GATCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT1340.27643115007735947No Hit
AGGGCCTGCCTTAAGTGTGAAAGTAATGACTGATGAAAGTGGAAAATCCA1310.2702423929860753No Hit
ACACATATGGCCTAGACTACGTACATAACCCAAACCTACTCCAATGCTAA1110.22898401237751417No Hit
GGCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACTA1030.21248066013408975No Hit
GATCCTCGTGGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACT1010.20835482207323364No Hit
GATCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACT1000.2062919030428056No Hit
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCGAGT960.19804022692109335No Hit
AATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA890.18359979370809695No Hit
TCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA750.15471892728210418No Hit
TCCCACAGGACAACCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC750.15471892728210418No Hit
ACACCCAGCCTTGGCTAGCTGGAACACCTGTCTCTTATACACATCTCCGA690.14234141309953585No Hit
TCCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACTA660.13615265600825166No Hit
TAGTGAGCATGTCCCCGCTGCAGCCCACCTCCAGTGTCATGCCCTCCAGG620.12790097988653945No Hit
TCCCACAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC590.12171222279525529No Hit
GTATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT570.11758638473439917No Hit
GATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA530.10933470861268695No Hit
GCCCTCGCTGTCACTTTCCTAGGTCTTCTAACAGCCCTAGACCTCAACTA510.10520887055183084No Hit
TCAGCGTGCTCTTGCTAAAACTGGAGCTGAATCTATCAGTTTGCTTGAGT490.10108303249097472No Hit
TCCCACAGGACAACCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTC490.10108303249097472No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)