FastQCFastQC Report
Sun 21 May 2023
EGAF00008003544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53915
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT2087138.71093387739961No Hit
GCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA908616.85245293517574No Hit
TGTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG42247.834554391171287No Hit
GCCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACTA42107.808587591579339No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAA34856.4638783269961975No Hit
TGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTG22394.152833163312622No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGTTGGA6651.2334229806176389No Hit
GCCCTACGCGCAGAGGAGCGCCGCCGCGGCGTACGGCCACCAGCCCATCA6051.1221366966521378No Hit
GGATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT4570.8476305295372345No Hit
GATCCTCGTGGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT3390.6287675044050821No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG3030.5619957340257813No Hit
ACACATATGGCCTAGACTACGTACATAACCCAAACCTACTCCAATGCTAA2820.5230455346378559No Hit
GATCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT2760.5119169062413057No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGACGGA2610.4840953352499304No Hit
TCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA2120.3932115366781044No Hit
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCGAGT1750.32458499489937864No Hit
TTTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTG1670.3097468237039785No Hit
GGCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA1470.2726513957154781No Hit
GATCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACT1440.267087081517203No Hit
TCCCACAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC1360.25224891032180285No Hit
AATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA1170.21700825373272745No Hit
TCCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACTA1110.20587962533617732No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATTCTAA980.18176759714365204No Hit
TCCCACAGGACAACCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC920.1706389687471019No Hit
GATCCTCGTGGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACT910.1687841973476769No Hit
ACACCCAGCCTTGGCTAGCTGGAACACCTGTCTCTTATACACATCTCCGA770.1428173977557266No Hit
AGGGCCTGCCTTAAGTGTGAAAGTAATGACTGATGAAAGTGGAAAATCCA750.13910785495687658No Hit
GGCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACTA740.13725308355745156No Hit
GATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA660.12241491236205136No Hit
TCCCACAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTC610.11314105536492627No Hit
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTG560.10386719836780117No Hit
TCCCACAGGACAACCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTC560.10386719836780117No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)