FastQCFastQC Report
Wed 31 May 2023
EGAF00008003557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71687
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC1414619.733005984348626No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT1303518.183213134878013No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG757310.563979522089081No Hit
GGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT37975.296636768172751No Hit
CAACGCGCTCACTTCCTTCGTGGACGCCAGCATGGTGTACGGCAGCGAGG13891.9375898001032263No Hit
GTGTTCCAGCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACT11361.5846666759663535No Hit
GGCTGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT5070.7072412013335752No Hit
GGCTGTGGGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT4540.6333086891626096No Hit
GGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC3960.5524014116924966No Hit
TGCGGCGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT3750.5231073974360763No Hit
TGGGTTTTGCCTGCATACGACAAACAGACCTAAAATCGCTCATTGCATAC2690.3752423730941454No Hit
AGCTCCATCTGCATACGACAAACAGACCTAAAATCGCTCATTGCATACTC2680.37384742003431587No Hit
TGCGGTGGGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT2550.3557130302565319No Hit
GTTTGGACCCCAGACCTGCTGAACAACCACATCTTGAAGTCAGCTATGTG2520.35152817107704326No Hit
GGCTGTGTGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT2330.325024062940282No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGAATGTTGGAGAAATAAAAT2260.3152593915214753No Hit
GTGTTCCAGCTGGACGAGGCCGACGAGGCGGCGGCTGCGGCAGCAGCGGC1830.25527640994880524No Hit
TGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC1760.24551173852999847No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATAGCATACTC1750.24411678547016893No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGTAATAAAAT1740.2427218324103394No Hit
GGCTGTGGGTGGTTGTGTTGATTCAAATTATGAGTTTTTTGGAAAGTCAT1720.2399319262906803No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGCAATAAAAT1560.21761267733340772No Hit
AGCTCCATCTGCCTACGAGAAACAGACCTAAAATCGCTCATTGCATACTC1410.1966883814359647No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATCGCATACTC1340.18692371001715793No Hit
CCGACCTGGCTTCCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG1270.17715903859835117No Hit
GGCTGCGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT1230.17157922635903303No Hit
AGCTCCAGCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC1230.17157922635903303No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGGTGGAGAAATAAAAT1220.17018427329920346No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGGAATAAAAT1210.16878932023937393No Hit
AGCTCCATCTGCCTACGACAAACAAACCTAAAATCGCTCATTGCATACTC1170.1632095080000558No Hit
TGCGGTGTGTGATGCTAGGGTAGAGTCCGAGTATGTTGGAGAAATAAAAT1150.16041960188039672No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAATTTAATCG1120.1562347427009081No Hit
TGCGGTGTGAGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT1040.14507511822227181No Hit
AGGTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC1000.13949530598295368No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAGAATCGCTCATTGCATACTC980.1367053998632946No Hit
GGCTGTGGGTGGTTGTGTTGATTAAAATTATGTGTTTTTTGGAAAGTCAT970.13531044680346507No Hit
AGCTCTATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC960.13391549374363554No Hit
CCGACCTGGCTTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG960.13391549374363554No Hit
TCCTTGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAA960.13391549374363554No Hit
TGCGGTGTGTGATGCTAGCGTAGAATCCGAGTATGGTGGAGAAATAAAAT940.13112558762397644No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGATTTTAATCG930.12973063456414693No Hit
TGCGGTGTGTGAAGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT900.1255457753846583No Hit
CCGACCTGGATTCCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG890.12415082232482878No Hit
AGCTCCATCTGCCTACGACAAACAGACCGAGAATCGCTCATTGCATACTC870.1213609162051697No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGGGTATGTTGGAGAAATAAAAT850.11857101008551062No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGAACACGTAGGACTTTAATCG840.1171760570256811No Hit
AGCTCCATCTGCCTACGACAAACAGCCCTAAAATCGCTCATTGCATACTC820.11438615090602201No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAGTTTAATCG820.11438615090602201No Hit
TGCGGTGTGTGATGCTAGCGTAGAATCCGAGTATGTTGGAGAAATAAAAT800.11159624478636293No Hit
GTGTTCCAGCTAGCCAAGGCTGGGTGTCTGTCTCTTATACACATCTGACG790.11020129172653341No Hit
TGCGGTGTGTGATGATAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT780.10880633866670386No Hit
AGCTCCATCTGTCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC770.10741138560687433No Hit
ACTCGCCATTCTCATCCAAACCCCCTGAAGCTTCACCGGCGCAGTCATTC770.10741138560687433No Hit
TCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG760.10601643254704478No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAAATCACGTAGGACTTTAATCG750.10462147948721526No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAACTCGCTCATTGCATACTC740.10322652642738572No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGAACACGTAGGACGTTAATCG720.10043662030772664No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)