FastQCFastQC Report
Wed 31 May 2023
EGAF00008003558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111084
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT3332930.003420834683663No Hit
GCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA2812925.322278635987182No Hit
TGTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG1516013.647329948507437No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAA78257.04421878938461No Hit
ACACATATGGCCTAGACTACGTACATAACCCAAACCTACTCCAATGCTAA46544.189622267833351No Hit
GCCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACTA23082.077706960498362No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGACGGA16711.5042670411580426No Hit
TGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTG13821.2441035612689495No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG12861.1576824745237837No Hit
GATCCTCGTGGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT8500.7651867055561556No Hit
GCCCTACGCGCAGAGGAGCGCCGCCGCGGCGTACGGCCACCAGCCCATCA5770.5194267401245904No Hit
GGATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT5250.47261531813762553No Hit
TAGTGAGCATGTCCCCGCTGCAGCCCACCTCCAGTGTCATGCCCTCCAGG4820.4339058730330201No Hit
TAGTGAGCATGTCCCCGCTGCAGCCCACCTCCAGTGTCGTGCCCTCCAGG4790.4312052140722336No Hit
ACACCCAGCCTTGGCTAGCTGGAACACCTGTCTCTTATACACATCTCCGA4410.39699686723560546No Hit
GATCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT3460.31147600014403515No Hit
GGCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA3340.3006733643008894No Hit
AGGGCCTGCCTTAAGTGTGAAAGTAATGACTGATGAAAGTGGAAAATCCA2850.25656260127471103No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATTCTAA2780.2502610636995427No Hit
TCAGCGTGCTCTTGCTAAAACTGGAGCTGAATCTATCAGTTTGCTTGAGT2530.22775557235965574No Hit
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCGAGT2490.22415469374527383No Hit
TCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA2370.21335205790212813No Hit
TCCCACAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC2060.18544524864066833No Hit
TCCCACAGGACAACCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC1820.16383997695437688No Hit
AATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA1780.16023909833999495No Hit
ACACATATGGCCTAGACTACGTACATAACCCAAACCTACTCCAATTCTAA1690.15213712145763567No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGTTTCGTTTG1640.14763602318965827No Hit
ACACCCAGCCTCGTCCAGCTGGAACACCTGTCTCTTATACACATCTCCGA1500.13503294803932162No Hit
TCTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG1470.13233228907853514No Hit
TTTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTG1360.12242987288898491No Hit
TCAGCGTGCTCTTGCTAAAACTAATCGTCCCAACAATTATATTACTACCA1330.11972921392819848No Hit
ACACCCAGCCTTGTCCAGCTGGAACACCTGTCTCTTATACACATCTCCGA1260.11342767635303012No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)