FastQCFastQC Report
Sun 21 May 2023
EGAF00008003578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100342
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT3069830.59337067230073No Hit
GCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA2295822.87975125072253No Hit
TGTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG1051610.480157860118396No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAA63366.314404735803551No Hit
GCCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACTA47974.7806501763967235No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGTTGGA29722.961870403220984No Hit
TGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTG27482.738633872157222No Hit
ACACATATGGCCTAGACTACGTACATAACCCAAACCTACTCCAATGCTAA23092.3011301349385103No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGACGGA22932.285184668433956No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG10331.029479181200295No Hit
GATCCTCGTGGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT6880.6856550596958402No Hit
GGATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT4160.4145821291184151No Hit
GATCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT4150.41358553746188037No Hit
TAGTGAGCATGTCCCCGCTGCAGCCCACCTCCAGTGTCATGCCCTCCAGG4120.4105957624922764No Hit
GCCCTACGCGCAGAGGAGCGCCGCCGCGGCGTACGGCCACCAGCCCATCA3950.39365370433118735No Hit
GCCCTACGCGCAGAGGAGCGCCGCCGCGGCGTACAGCCACCAGCCCATCA3550.35379003806980125No Hit
TTTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTG3180.3169161467780192No Hit
TAGTGAGCATGTCCCCGCTGCAGCCCACCTCCAGTGTCGTGCCCTCCAGG3080.30695023021267265No Hit
GGCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA2870.286021805425445No Hit
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCGAGT2560.2551274640728708No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATTCTAA2310.23021267265950449No Hit
ACACCCAGCCTTGGCTAGCTGGAACACCTGTCTCTTATACACATCTCCGA2260.22522971437683123No Hit
TCAGCGTGCTCTTGCTAAAACTGGAGCTGAATCTATCAGTTTGCTTGAGT2150.21426720615495007No Hit
TCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA2090.20828765621574216No Hit
AGGGCCTGCCTTAAGTGTGAAAGTAATGACTGATGAAAGTGGAAAATCCA1840.18337286480237588No Hit
TCCCACAGGACAACCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC1780.17739331486316795No Hit
AATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA1670.1664308066412868No Hit
TCCCACAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC1650.16443762332821749No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGTTTCGTTTG1210.12058759044069284No Hit
CCGTTTTCTTGTAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGTTGGA1040.10364553227960377No Hit
TCAGCGTGCTCTTGCTAAAACTAATCGTCCCAACAATTATATTACTACCA1040.10364553227960377No Hit
GATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA1020.10165234896653445No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)