FastQCFastQC Report
Sun 21 May 2023
EGAF00008003583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142454
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC2834219.895545228635207No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT2530817.765734903898803No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG1606211.275218667078496No Hit
GGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT81425.715529223468629No Hit
CAACGCGCTCACTTCCTTCGTGGACGCCAGCATGGTGTACGGCAGCGAGG36192.540469204093953No Hit
GTGTTCCAGCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACT23311.6363176885169948No Hit
GTGTTCCAGCTGGACGAGGCCGACGAGGCGGCGGCTGCGGCAGCAGCGGC9410.6605641119238491No Hit
TGCGGCGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT8830.6198492144832718No Hit
GGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC8530.5987897847726285No Hit
GGCTGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT7970.5594788493127606No Hit
GGCTGTGGGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT7460.5236778188046668No Hit
TGGGTTTTGCCTGCATACGACAAACAGACCTAAAATCGCTCATTGCATAC5430.38117567776264616No Hit
GTTTGGACCCCAGACCTGCTGAACAACCACATCTTGAAGTCAGCTATGTG5400.37906973479158185No Hit
TGCGGTGGGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT5400.37906973479158185No Hit
GGCTGTGTGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT5310.3727519058783888No Hit
AGCTCCATCTGCATACGACAAACAGACCTAAAATCGCTCATTGCATACTC4290.3011498448622011No Hit
TGTACCCCCTTCTTCCTGCTGAATACAGACCACTTACTTTCTGTGCATCT4240.29763993991042725No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGAATGTTGGAGAAATAAAAT3530.24779928959523775No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGTAATAAAAT3330.23375966978814214No Hit
AGCTCCAGCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC3330.23375966978814214No Hit
AGCTCCATCTGCCTACGAGAAACAGACCTAAAATCGCTCATTGCATACTC3260.22884580285565864No Hit
TGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC3120.21901806899069176No Hit
CCGACCTGGCTTCCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG3110.21831608800033697No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATAGCATACTC3060.21480618304856305No Hit
GGCTGTGGGTGGTTGTGTTGATTCAAATTATGAGTTTTTTGGAAAGTCAT2730.19164081036685526No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATCGCATACTC2690.18883288640543613No Hit
GGCTGCGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT2520.17689920956940486No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGCAATAAAAT2520.17689920956940486No Hit
GGCTGTGGGTGGTTGTGTTGATTAAAATTATGTGTTTTTTGGAAAGTCAT2360.16566751372372837No Hit
TGCGGTGTGTGATGCTAGGGTAGAGTCCGAGTATGTTGGAGAAATAAAAT2260.15864770382018054No Hit
CAACGCGCTCACTTCCTTCGTGGACGCCAGCATGGTGTATGGCAGCGAGG2230.15654176084911622No Hit
AGCTCCATCTGCCTACGACAAACAAACCTAAAATCGCTCATTGCATACTC2160.15162789391663273No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGGTGGAGAAATAAAAT2140.15022393193592318No Hit
TGCGGTGTGAGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT2110.14811798896485884No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGGAATAAAAT2090.14671402698414926No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAATTTAATCG2030.14250214104202058No Hit
AGCTCCATCTGCCTACGACAAACAGACCGAGAATCGCTCATTGCATACTC2010.141098179061311No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAGAATCGCTCATTGCATACTC1940.13618431212882756No Hit
CCGACCTGGATTCCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG1900.1333763881674084No Hit
TGCGGTGTGTGATGCTAGCGTAGAATCCGAGTATGGTGGAGAAATAAAAT1840.12916450222527973No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAGTTTAATCG1810.1270585592542154No Hit
CCGACCTGGCTTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG1780.12495261628315106No Hit
GTGTTCCAGCTAGCCAAGGCTGGGTGTCTGTCTCTTATACACATCTGACG1780.12495261628315106No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAAATCACGTAGGACTTTAATCG1730.12144271133137714No Hit
TGCGGTGTGTGATGCTAGCGTAGAATCCGAGTATGTTGGAGAAATAAAAT1710.12003874935066759No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGAACACGTAGGACTTTAATCG1700.11933676836031279No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGGGTATGTTGGAGAAATAAAAT1690.11863478736995803No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGATTTTAATCG1680.11793280637960324No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATGGCATACTC1640.11512488241818411No Hit
AGCTCCATCTGTCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC1630.11442290142782932No Hit
TGCGGTGTGTGATGCTAGGGTAGCATCCGAGTATGTTGGAGAAATAAAAT1630.11442290142782932No Hit
GGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT1600.112316958456765No Hit
AGCTCTATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC1480.10389318657250761No Hit
ACTCGCCATTCTCATCCAAACCCCCTGAAGCTTCACCGGCGCAGTCATTC1470.10319120558215285No Hit
AGCTCCATCTGACTACGACAAACAGACCTAAAATCGCTCATTGCATACTC1440.1010852626110885No Hit
AGCTCCATCTGCCTACGACAAACAGCCCTAAAATCGCTCATTGCATACTC1430.1003832816207337No Hit
GTTTTCCAGCTGCCTACGACAAACAGACCTAAACTCGCTCATTGCATACT1430.1003832816207337No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)