FastQCFastQC Report
Sun 21 May 2023
EGAF00008003586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88532
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT3155635.64360909049835No Hit
GCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA2333926.36221931053179No Hit
TGTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG1118812.637238512628201No Hit
GCCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACTA29243.302760583743731No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGTTGGA17461.9721682555460172No Hit
TGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTG15521.7530384493742377No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAA15071.7022093706230517No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG9781.104685311525776No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGACGGA9261.0459494871910722No Hit
GATCCTCGTGGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT8861.0007680838566846No Hit
GCCCTACGCGCAGAGGAGCGCCGCCGCGGCGTACGGCCACCAGCCCATCA7070.7985813039353004No Hit
GATCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT5220.5896173135137578No Hit
ACACATATGGCCTAGACTACGTACATAACCCAAACCTACTCCAATGCTAA5060.5715447521800027No Hit
GCCCTACGCGCAGAGGAGCGCCGCCGCGGCGTACAGCCACCAGCCCATCA4460.5037726471784213No Hit
GGCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA3080.34789680567478426No Hit
GGATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT3040.34337866534134553No Hit
TCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA2890.3264356390909502No Hit
ACACCCAGCCTTGGCTAGCTGGAACACCTGTCTCTTATACACATCTCCGA2870.3241765689242308No Hit
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCGAGT2780.3140107531739936No Hit
TCCCACAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC2360.26657027967288666No Hit
TCCCACAGGACAACCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC2110.23833190258889442No Hit
AATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA1730.19540956942122623No Hit
TTTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTG1450.16378258708715493No Hit
TAGTGAGCATGTCCCCGCTGCAGCCCACCTCCAGTGTCGTGCCCTCCAGG1450.16378258708715493No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGTTTCGTTTG1250.14119188541996114No Hit
AGGGCCTGCCTTAAGTGTGAAAGTAATGACTGATGAAAGTGGAAAATCCA1080.12198978900284643No Hit
ACACCCAGCCTCGTCCAGCTGGAACACCTGTCTCTTATACACATCTCCGA950.10730583291917047No Hit
GATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA930.10504676275245109No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)