FastQCFastQC Report
Wed 31 May 2023
EGAF00008003589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73615
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC1490620.248590640494466No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT1335818.145758337295387No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG757410.288663995109692No Hit
GGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT33024.48549887930449No Hit
GTGTTCCAGCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACT15432.096040209196495No Hit
CAACGCGCTCACTTCCTTCGTGGACGCCAGCATGGTGTACGGCAGCGAGG11891.6151599538137607No Hit
GGCTGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT8321.1302044420294777No Hit
GGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC6980.948176322760307No Hit
GGCTGTGGGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT6600.8965564083406914No Hit
TGCGGCGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT4480.6085716226312573No Hit
TGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC3170.4306187597636351No Hit
TGCGGTGGGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT3050.4143177341574407No Hit
TGGGTTTTGCCTGCATACGACAAACAGACCTAAAATCGCTCATTGCATAC2960.4020919649527949No Hit
GTGTTCCAGCTGGACGAGGCCGACGAGGCGGCGGCTGCGGCAGCAGCGGC2600.3531888881342118No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGAATGTTGGAGAAATAAAAT2200.2988521361135638No Hit
TCCTTGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAA2180.2961352985125314No Hit
AGCTCCATCTGCATACGACAAACAGACCTAAAATCGCTCATTGCATACTC2060.279834272906337No Hit
GGCTGTGTGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT2020.2744005977042722No Hit
GTTTGGACCCCAGACCTGCTGAACAACCACATCTTGAAGTCAGCTATGTG1910.25945799089859406No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGTAATAAAAT1820.24723222169394823No Hit
CCGACCTGGCTTCCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG1750.23772329009033483No Hit
AGCTCCATCTGCCTACGAGAAACAGACCTAAAATCGCTCATTGCATACTC1700.23093119608775386No Hit
AGCTCCAGCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC1600.21734700808259186No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATAGCATACTC1460.1983291448753651No Hit
TGCGGTGTGTGATGCTAGGGTAGAGTCCGAGTATGTTGGAGAAATAAAAT1360.1847449568702031No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGGGTATGTTGGAGAAATAAAAT1280.1738776064660735No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGCAATAAAAT1200.1630102560619439No Hit
GGCTGTGGGTGGTTGTGTTGATTCAAATTATGAGTTTTTTGGAAAGTCAT1180.1602934184609115No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATCGCATACTC1140.15485974325884672No Hit
AGCTCCATCTGCCTACGACAAACAAACCTAAAATCGCTCATTGCATACTC1130.1535013244583305No Hit
CCGACCTGGATTCCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG1090.1480676492562657No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGGTGGAGAAATAAAAT1090.1480676492562657No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAGTTTAATCG1070.1453508116552333No Hit
GTTTTCCAGCTGCCTACGACAAACAGACCTAAACTCGCTCATTGCATACT1040.1412755552536847No Hit
GTGTTCCAGCTAGCCAAGGCTGGGTGTCTGTCTCTTATACACATCTGACG1020.13855871765265232No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAGAATCGCTCATTGCATACTC1010.1372002988521361No Hit
AGCTCCATCTGCCTACGACAAACAGACCGAGAATCGCTCATTGCATACTC1000.13584188005161993No Hit
GGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT1000.13584188005161993No Hit
GGCTGCGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT990.13448346125110372No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGGAATAAAAT980.1331250424505875No Hit
TGCGGTGTGTGATGCTAGCGTAGAATCCGAGTATGGTGGAGAAATAAAAT970.13176662365007133No Hit
GGCTGTGGGTGGTTGTGTTGATTAAAATTATGTGTTTTTTGGAAAGTCAT950.12904978604903894No Hit
TGCGGTGTGTGTTGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT920.12497452964749033No Hit
AGCTCCATCTGTCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC920.12497452964749033No Hit
TGCGGTGTGTGATGCTAGCGTAGAATCCGAGTATGTTGGAGAAATAAAAT910.12361611084697412No Hit
TCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG910.12361611084697412No Hit
ACTCGCCATTCTCATCCAAACCCCCTGAAGCTTCACCGGCGCAGTCATTC910.12361611084697412No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATGGCATACTC880.11954085444542553No Hit
TGCGGTGTGAGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT870.11818243564490932No Hit
TGCGGTGTGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT840.11410717924336072No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGAACACGTAGGACTTTAATCG840.11410717924336072No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAATTTAATCG820.11139034164232833No Hit
TGCGGTGTGTGATGCTAGGGTAGCATCCGAGTATGTTGGAGAAATAAAAT780.10595666644026354No Hit
TGCGGTGTGTGCTGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT770.10459824763974733No Hit
CCGACCTGGCTTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG770.10459824763974733No Hit
GTGTTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACT770.10459824763974733No Hit
AGCTCCATCTGCCTACGACAAACAGTCCTAAAATCGCTCATTGCATACTC770.10459824763974733No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)