FastQCFastQC Report
Wed 31 May 2023
EGAF00008003600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94239
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT3127533.18689714449432No Hit
GCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA2258423.964600643045873No Hit
TGTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG999210.602828977387281No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAA52395.5592695168666895No Hit
GCCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACTA29983.181273145937457No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGTTGGA25732.7302921295854157No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGACGGA19552.0745126752193888No Hit
ACACATATGGCCTAGACTACGTACATAACCCAAACCTACTCCAATGCTAA17591.8665308417958593No Hit
TGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTG16841.7869459565572638No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG8400.8913507146722695No Hit
GATCCTCGTGGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT7140.7576481074714291No Hit
GCCCTACGCGCAGAGGAGCGCCGCCGCGGCGTACAGCCACCAGCCCATCA6700.710958308131453No Hit
GCCCTACGCGCAGAGGAGCGCCGCCGCGGCGTACGGCCACCAGCCCATCA5150.5464828786383557No Hit
GATCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT4400.4668979933997602No Hit
GGATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT3550.37670179012935195No Hit
GGCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA3420.36290707668799543No Hit
TAGTGAGCATGTCCCCGCTGCAGCCCACCTCCAGTGTCGTGCCCTCCAGG3300.35017349504982015No Hit
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCGAGT2460.2610384235825932No Hit
AGGGCCTGCCTTAAGTGTGAAAGTAATGACTGATGAAAGTGGAAAATCCA2280.2419380511253303No Hit
TCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA2150.2281433376839737No Hit
TCAGCGTGCTCTTGCTAAAACTGGAGCTGAATCTATCAGTTTGCTTGAGT2100.22283767866806736No Hit
TAGTGAGCATGTCCCCGCTGCAGCCCACCTCCAGTGTCATGCCCTCCAGG1910.20267617440762317No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATTCTAA1820.1931259881789917No Hit
TCCCACAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC1770.18782032916308536No Hit
TCCCACAGGACAACCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC1750.1856980655567228No Hit
ACACCCAGCCTTGGCTAGCTGGAACACCTGTCTCTTATACACATCTCCGA1640.17402561572172878No Hit
AATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA1570.1665976930994599No Hit
TTTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTG1330.14113052982310934No Hit
GATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA1130.11990789375948387No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGTTTCGTTTG1080.1146022347435775No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)