FastQCFastQC Report
Sun 21 May 2023
EGAF00008003612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95859
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT2808429.297196924649743No Hit
GCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA2637627.51541326323037No Hit
TGTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG1302613.588708415485243No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAA1146711.962361384950814No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGTTGGA12521.3060849789795428No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG11161.1642099333395926No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGACGGA8130.8481206772447032No Hit
GATCCTCGTGGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT8060.8408182851897057No Hit
GCCCTACGCGCAGAGGAGCGCCGCCGCGGCGTACGGCCACCAGCCCATCA7840.8178679101597138No Hit
TAGTGAGCATGTCCCCGCTGCAGCCCACCTCCAGTGTCGTGCCCTCCAGG4520.47152588697983494No Hit
GGATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT4450.46422349492483755No Hit
GGCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA4040.4214523414598525No Hit
TCAGCGTGCTCTTGCTAAAACTGGAGCTGAATCTATCAGTTTGCTTGAGT3950.4120635516748558No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATTCTAA3900.4068475573498576No Hit
GATCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT3670.382853983454866No Hit
AGGGCCTGCCTTAAGTGTGAAAGTAATGACTGATGAAAGTGGAAAATCCA3390.3536444152348762No Hit
ACACCCAGCCTTGGCTAGCTGGAACACCTGTCTCTTATACACATCTCCGA2980.31087326176989116No Hit
TAGTGAGCATGTCCCCGCTGCAGCCCACCTCCAGTGTCATGCCCTCCAGG2880.3004412731198948No Hit
TCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA2240.23367654575991822No Hit
TCCCACAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC1990.20759657413492735No Hit
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCGAGT1980.2065533752699277No Hit
TCCCACAGGACAACCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC1900.19820778434993064No Hit
AGACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAA1330.13874544904495142No Hit
TCAGCGTGCTCTTGCTAAAACTAATCGTCCCAACAATTATATTACTACCA1330.13874544904495142No Hit
AATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA1260.131443056989954No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGTTTCGTTTG1240.12935665925995474No Hit
ACACCCAGCCTCGTCCAGCTGGAACACCTGTCTCTTATACACATCTCCGA1060.11057907968996131No Hit
ACACATATGGCCTAGACTACGTACATACCCTAAACCTACTCCAATTCTAA1030.10744948309496238No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)