FastQCFastQC Report
Sun 21 May 2023
EGAF00008003615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97995
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC1722717.579468340221442No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT1701517.36313077197816No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG95859.781111281187815No Hit
GGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT78768.03714475228328No Hit
CAACGCGCTCACTTCCTTCGTGGACGCCAGCATGGTGTACGGCAGCGAGG42974.384917597836624No Hit
GTGTTCCAGCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACT17351.7704984948211644No Hit
GGCTGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT8470.8643298127455482No Hit
GTTTGGACCCCAGACCTGCTGAACAACCACATCTTGAAGTCAGCTATGTG7720.7877952956783509No Hit
GGCTGTGGGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT7250.7398336649829073No Hit
GTGTTCCAGCTGGACGAGGCCGACGAGGCGGCGGCTGCGGCAGCAGCGGC6750.6888106536047757No Hit
GGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC6460.6592173070054594No Hit
TGCGGCGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT4600.46941170467881016No Hit
GGCTGTGTGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT4170.425531914893617No Hit
TGTACCCCCTTCTTCCTGCTGAATACAGACCACTTACTTTCTGTGCATCT3910.39899994897698865No Hit
TCCTTGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAA3400.3469564773712944No Hit
TGGGTTTTGCCTGCATACGACAAACAGACCTAAAATCGCTCATTGCATAC3330.3398132557783561No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGAATGTTGGAGAAATAAAAT2980.304097147813664No Hit
TGCGGTGGGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT2960.3020562273585387No Hit
TGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC2570.2622582784835961No Hit
GGCTGTGGGTGGTTGTGTTGATTCAAATTATGAGTTTTTTGGAAAGTCAT2390.24388999438746875No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGTAATAAAAT2330.23776723302209296No Hit
GGCTGTGGGTGGTTGTGTTGATTAAAATTATGTGTTTTTTGGAAAGTCAT2310.2357263125669677No Hit
AGCTCCATCTGCATACGACAAACAGACCTAAAATCGCTCATTGCATACTC2200.22450125006377875No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGCAATAAAAT2120.21633756824327774No Hit
GGCTGCGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT2090.2132761875605898No Hit
AGAACTTTGCGGCAGCTTGTTTTCTGTTCGTATTTCGACGTAACTCAAGC1910.19490790346446246No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATAGCATACTC1870.19082606255421194No Hit
CCGACCTGGCTTCCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG1710.17449869891320985No Hit
AGCTCCATCTGCCTACGAGAAACAGACCTAAAATCGCTCATTGCATACTC1560.1591917954997704No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGGTGGAGAAATAAAAT1450.14796673299658145No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGGAATAAAAT1430.1459258125414562No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATCGCATACTC1410.14388489208633093No Hit
TGCGGTGTGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT1390.14184397163120568No Hit
GGCTGTGGGTGGTTGTGTTGATTCAAATTATATGTTTTTTGGAAAGTCAT1390.14184397163120568No Hit
CAACGCGCTCACTTCCTTCGTGGACGCCCTCATGGTGTACGGCAGCGAGT1360.13878259094851778No Hit
TGCGGTGTGTGATGCTAGCGTAGAATCCGAGTATGGTGGAGAAATAAAAT1350.13776213072095514No Hit
AGAACTTTGCGGCAGCTTGTTTTCTGTTCGTATTTCGACATAACTCAAGC1330.1357212102658299No Hit
TGCGGTGTGTGATGCTAGGGTAGAGTCCGAGTATGTTGGAGAAATAAAAT1310.13368028981070462No Hit
AGCTCCAGCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC1290.13163936935557938No Hit
AGCTCCATCTGCCTACGACAAACAAACCTAAAATCGCTCATTGCATACTC1240.1265370682177662No Hit
AGCTCCATCTGCCTACGACAAACAGACCGAGAATCGCTCATTGCATACTC1200.1224552273075157No Hit
CAACGCGCTCACTTCCTTCGTGGACGCCCGCATGGTGTACGGCAGCGAGG1170.11939384662482778No Hit
GTGTTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACT1160.11837338639726516No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAGAATCGCTCATTGCATACTC1140.1163324659421399No Hit
GGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGTAAAGTCAT1130.11531200571457728No Hit
TGCGGTGTGTGATGCTAGCGTAGAATCCGAGTATGTTGGAGAAATAAAAT1130.11531200571457728No Hit
CCGACCTGGCTTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG1130.11531200571457728No Hit
TGCGGTGTGTGAAGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT1100.11225062503188937No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAATTTAATCG1070.10918924434920149No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAGTTTAATCG1060.10816878412163887No Hit
ACTCGCCATTCTCATCCAAACCCCCTGAAGCTTCACCGGCGCAGTCATTC1060.10816878412163887No Hit
TCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG1030.10510740343895096No Hit
CAACGCGCTCACTTCCTTCGTGGACGCCAGCAAGGTGTACGGCAGCGAGG1020.10408694321138834No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAACCG1020.10408694321138834No Hit
TGCGGTGTGAGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT1010.1030664829838257No Hit
GTTTTCCAGCTGCCTACGACAAACAGACCTAAACTCGCTCATTGCATACT1000.10204602275626308No Hit
CAACGCGCTCACTTCCTTCGTGGACGCCAGCATGGTGTACGTCAGCGAGG990.10102556252870046No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGGGTATGTTGGAGAAATAAAAT990.10102556252870046No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATGGCATACTC980.10000510230113781No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)