FastQCFastQC Report
Sun 21 May 2023
EGAF00008003616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77421
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT2297529.67541106418155No Hit
GCCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACTA1140614.732436935715116No Hit
GCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA968512.509525839242583No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAA74349.602045956523424No Hit
TGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTG58607.569005825292879No Hit
TGTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG44945.804626651683652No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGACGGA15892.0524147195205433No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGTTGGA8881.1469756267679312No Hit
GCCCTACGCGCAGAGGAGCGCCGCCGCGGCGTACGGCCACCAGCCCATCA7961.0281448185892716No Hit
ACACATATGGCCTAGACTACGTACATAACCCAAACCTACTCCAATGCTAA7700.994562198886607No Hit
GCCCTACGCGCAGAGGAGCGCCGCCGCGGCGTACAGCCACCAGCCCATCA5550.716859766729957No Hit
TTTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTG5100.6587360018599605No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG4050.5231138838299686No Hit
GGATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT3300.42624094237997445No Hit
GATCCTCGTGGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT3200.4133245501866419No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATTCTAA2690.34745095000064585No Hit
GATCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACT2690.34745095000064585No Hit
GATCCTCGTGGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACT2410.31128505185931465No Hit
GATCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT2180.28157734981464977No Hit
TCAGCGTGCTCTTGCTAAAACTGGAGCTGAATCTATCAGTTTGCTTGAGT2030.26220276152465094No Hit
AGGGCCTGCCTTAAGTGTGAAAGTAATGACTGATGAAAGTGGAAAATCCA1810.23378669869931928No Hit
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCGAGT1710.22087030650598674No Hit
TAGTGAGCATGTCCCCGCTGCAGCCCACCTCCAGTGTCATGCCCTCCAGG1660.21441211040932046No Hit
GGCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACTA1630.21053719275132068No Hit
TAGTGAGCATGTCCCCGCTGCAGCCCACCTCCAGTGTCGTGCCCTCCAGG1530.19762080055798814No Hit
GGCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA1410.18212112992598906No Hit
ACACCCAGCCTTGGCTAGCTGGAACACCTGTCTCTTATACACATCTCCGA1300.16791309851332326No Hit
TCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA1240.1601632631973237No Hit
AATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA1230.15887162397799048No Hit
TCCCACAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTC1150.14853851022332443No Hit
TCCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACTA1150.14853851022332443No Hit
TCCCACAGGACAACCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTC1030.13303883959132534No Hit
TCCCACAGGACAACCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC990.12787228271399234No Hit
TCAGCGTGCTCTTGCTAAAACTAATCGTCCCAACAATTATATTACTACCA980.12658064349465906No Hit
AGACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAA920.11883080817865954No Hit
GCCCTCGCTGTCACTTTCCTAGGTCTTCTAACAGCCCTAGACCTCAACTA870.11237261208199327No Hit
TCCCACAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC820.10591441598532698No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)