FastQCFastQC Report
Wed 31 May 2023
EGAF00008003622

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003622
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88877
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT3323337.392126196878834No Hit
GCCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACTA1722719.382967471899367No Hit
TGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTG83849.433261698752208No Hit
GCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA79648.960698493423495No Hit
TGTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG45535.122810175861021No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAA39024.390337207601517No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGTTGGA9831.1060229305669633No Hit
TTTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTG7640.8596149735027059No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGACGGA6530.7347232692372605No Hit
GGATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT4650.5231949773282176No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG4150.46693745288432326No Hit
GATCCTCGTGGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACT3920.4410589916401319No Hit
GATCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACT3070.34542120008551147No Hit
ACACCCAGCCTTGGCTAGCTGGAACACCTGTCTCTTATACACATCTCCGA2700.3037906319970296No Hit
GATCCTCGTGGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT2600.29253912710825075No Hit
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCGAGT2450.27566186977508245No Hit
GGCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACTA2380.2677858163529372No Hit
GCCCTACGCGCAGAGGAGCGCCGCCGCGGCGTACGGCCACCAGCCCATCA2120.23853190364211213No Hit
TAGTGAGCATGTCCCCGCTGCAGCCCACCTCCAGTGTCGTGCCCTCCAGG1880.21152829190904282No Hit
AATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA1820.2047773889757755No Hit
TCCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACTA1630.18339952968709566No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATTCTAA1500.1687725733316831No Hit
GATCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT1490.1676474228428052No Hit
TCCCACAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTC1490.1676474228428052No Hit
TCCCACAGGACAACCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTGTTC1460.16427197137617158No Hit
GCCCTCGCTGTCACTTTCCTAGGTCTTCTAACAGCCCTAGACCTCAACTA1420.15977136942066No Hit
GATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA1220.13726835964310227No Hit
GGCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA1170.13164260719871285No Hit
ACACCCAGCCTTGTCCAGCTGGAACACCTGTCTCTTATACACATCTCCGA1150.12939230622095707No Hit
TTTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGTTTCGTTTG1050.11814080133217818No Hit
ACACCCAGCCTCGTCCAGCTGGAACACCTGTCTCTTATACACATCTCCGA1020.1147653498655445No Hit
TGTTGGATCAGGACATCCTGATGGTGCAACCGCTATCAAAGGTTCGTTTG1010.11364019937666664No Hit
GAATGACTGAGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT910.10238869448788775No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)