FastQCFastQC Report
Wed 31 May 2023
EGAF00008003643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84100
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC1487717.689655172413794No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT1383616.451843043995243No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG856410.183115338882283No Hit
GGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT64247.638525564803805No Hit
CAACGCGCTCACTTCCTTCGTGGACGCCAGCATGGTGTACGGCAGCGAGG22442.668252080856124No Hit
GTGTTCCAGCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACT11881.4126040428061832No Hit
TGCGGCGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT5120.6087990487514863No Hit
GTTTGGACCCCAGACCTGCTGAACAACCACATCTTGAAGTCAGCTATGTG4590.5457788347205708No Hit
GGCTGTGTGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT4460.5303210463733651No Hit
GGCTGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT4000.47562425683709864No Hit
GGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC3950.469678953626635No Hit
TGTACCCCCTTCTTCCTGCTGAATACAGACCACTTACTTTCTGTGCATCT3770.44827586206896547No Hit
GGCTGTGGGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT3620.43043995243757427No Hit
TGCGGTGGGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT3040.361474435196195No Hit
TGGGTTTTGCCTGCATACGACAAACAGACCTAAAATCGCTCATTGCATAC2670.3174791914387634No Hit
GGCTGTGGGTGGTTGTGTTGATTCAAATTATGAGTTTTTTGGAAAGTCAT2430.28894173602853745No Hit
AGCTCCATCTGCATACGACAAACAGACCTAAAATCGCTCATTGCATACTC2330.27705112960761No Hit
AGAACTTTGCGGCAGCTTGTTTTCTGTTCGTATTTCGACATAACTCAAGC2180.2592152199762188No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGAATGTTGGAGAAATAAAAT2180.2592152199762188No Hit
GGCTGCGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT2050.2437574316290131No Hit
GGCTGTGGGTGGTTGTGTTGATTAAAATTATGTGTTTTTTGGAAAGTCAT1990.23662306777645659No Hit
AGCTCCATCTGCCTACGAGAAACAGACCTAAAATCGCTCATTGCATACTC1750.2080856123662307No Hit
CCGACCTGGCTTCCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG1640.19500594530321044No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGTAATAAAAT1570.18668252080856124No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGCAATAAAAT1530.18192627824019025No Hit
AGCTCCAGCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC1460.17360285374554102No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATAGCATACTC1400.16646848989298454No Hit
AGAACTTTGCGGCAGCTTGTTTTCTGTTCGTATTTCGACGTAACTCAAGC1390.16527942925089179No Hit
TGCGGTGTGTGATGCTAGCGTAGAATCCGAGTATGGTGGAGAAATAAAAT1350.1605231866825208No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATCGCATACTC1350.1605231866825208No Hit
TGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC1350.1605231866825208No Hit
TGCGGTGTGTGATGCTAGGGTAGAGTCCGAGTATGTTGGAGAAATAAAAT1320.15695600475624255No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGGTGGAGAAATAAAAT1290.15338882282996433No Hit
AGCTCCATCTGCCTACGACAAACAAACCTAAAATCGCTCATTGCATACTC1250.14863258026159334No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGGAATAAAAT1190.14149821640903687No Hit
GGCTGTGGGTGGTTGTGTTGATTCAAATTATATGTTTTTTGGAAAGTCAT1140.13555291319857313No Hit
CCGACCTGGATTCCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG1100.13079667063020214No Hit
CCGACCTGGCTTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG1090.1296076099881094No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAATTTAATCG1080.12841854934601662No Hit
TCCTTGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAA1040.12366230677764566No Hit
GGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGTAAAGTCAT990.11771700356718191No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGATTTTAATCG990.11771700356718191No Hit
TGCGGTGTGAGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT950.11296076099881094No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGAACACGTAGGACGTTAATCG940.1117717003567182No Hit
CCGCCCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG930.11058263971462545No Hit
TGCGGTGTGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT910.10820451843043995No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAACCG910.10820451843043995No Hit
GTGTTCCAGCTAGCCAAGGCTGGGTGTCTGTCTCTTATACACATCTGACG910.10820451843043995No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAGTTTAATCG900.1070154577883472No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGGGTATGTTGGAGAAATAAAAT880.10463733650416171No Hit
AGCTCTATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC870.10344827586206896No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAGAATCGCTCATTGCATACTC860.10225921521997622No Hit
GGCTGTGGGTGGATGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT850.10107015457788349No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)