FastQCFastQC Report
Sun 21 May 2023
EGAF00008003644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85793
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT2658230.983879803713588No Hit
GCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA1932322.522816546804517No Hit
TGTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG1062112.379797885608383No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAA50575.894420290699707No Hit
ACACATATGGCCTAGACTACGTACATAACCCAAACCTACTCCAATGCTAA42794.987586399822829No Hit
GCCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACTA25522.974601657477883No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGTTGGA19162.2332824356299463No Hit
TGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTG15681.8276549368829627No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGACGGA12831.4954600025643119No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG12371.4418425745690209No Hit
GATCCTCGTGGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT5180.6037788630774072No Hit
TCAGCGTGCTCTTGCTAAAACTGGAGCTGAATCTATCAGTTTGCTTGAGT4950.5769701490797617No Hit
GCCCTACGCGCAGAGGAGCGCCGCCGCGGCGTACAGCCACCAGCCCATCA4420.5151935472591004No Hit
GGATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT3850.4487545603953703No Hit
AGGGCCTGCCTTAAGTGTGAAAGTAATGACTGATGAAAGTGGAAAATCCA3260.37998438101010573No Hit
GGCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA2790.32520135675404754No Hit
GCCCTACGCGCAGAGGAGCGCCGCCGCGGCGTACGGCCACCAGCCCATCA2470.2879022764094973No Hit
ACACCCAGCCTTGGCTAGCTGGAACACCTGTCTCTTATACACATCTCCGA2390.2785775063233597No Hit
GATCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT2170.25293438858648143No Hit
TTTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTG1810.2109729231988624No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATTCTAA1770.2063105381557936No Hit
AATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA1570.1829986129404497No Hit
ACACATATGGCCTAGACTACGTACATAACCCAAACCTACTCCAATTCTAA1540.17950182415814808No Hit
TAGTGAGCATGTCCCCGCTGCAGCCCACCTCCAGTGTCATGCCCTCCAGG1450.16901145781124335No Hit
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCGAGT1410.16434907276817456No Hit
TAGTGAGCATGTCCCCGCTGCAGCCCACCTCCAGTGTCGTGCCCTCCAGG1290.15036191763896822No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGTTTCGTTTG1260.14686512885666664No Hit
TCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA1240.14453393633513226No Hit
TCCCACAGGACAACCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC1110.12938118494515868No Hit
TCAGCGTGCTCTTGCTAAAACTAATCGTCCCAACAATTATATTACTACCA910.10606925972981478No Hit
TCCCACAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTGTTC860.1002412784259788No Hit
GATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA860.1002412784259788No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)