FastQCFastQC Report
Sun 21 May 2023
EGAF00008003654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115763
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT3755132.437825557388805No Hit
GCCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA2494121.544880488584436No Hit
TGTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG1697814.666171401915983No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGTTGGA50814.389139880618159No Hit
GCCCTCGCTGTCACTTTCCTAGGGCTTCTAACAGCCCTAGACCTCAACTA41633.596140390280141No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATGCTAA38443.320577386557017No Hit
TGTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTG36523.154721284002661No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGGTTCGTTTG19561.6896590447725093No Hit
CCGTTGTCTTGGAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGACGGA11991.035736807097259No Hit
TAGTGAGCATGTCCCCGCTGCAGCCCACCTCCAGTGTCATGCCCTCCAGG10480.9052978931091973No Hit
ACACCCAGCCTTGGCTAGCTGGAACACCTGTCTCTTATACACATCTCCGA9800.8465571901211959No Hit
TAGTGAGCATGTCCCCGCTGCAGCCCACCTCCAGTGTCGTGCCCTCCAGG9160.7912718226030769No Hit
GATCCTCGTGGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT5620.48547463351848175No Hit
GGATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTT4960.42846159826542163No Hit
TTTTGGATCAGGACATCCCGATGGTGCAGCCGCTATTAAAGGTTCGTTTG4070.3515803840605375No Hit
GGCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACTA3240.279882173060477No Hit
ACACCCAGCCTCGTCCAGCTGGAACACCTGTCTCTTATACACATCTCCGA3230.2790183391930064No Hit
ACACCCAGCCTTGTCCAGCTGGAACACCTGTCTCTTATACACATCTCCGA2880.24878415383153513No Hit
GAATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCGAGT1950.16844760415676857No Hit
TTTTGGATCAGGACATCCCGATGGTGCACCCGCTATTAAAGTTTCGTTTG1860.16067309934953308No Hit
AATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA1820.15721776387965067No Hit
ACACATATGGCCTAGACTACGTACATAACCCAAACCTACTCCAATGCTAA1810.15635393001218006No Hit
CCGTTTTCTTGTAAGCGCTGGTTGACGGCCAGCAGCCCCAGCTGGTTGGA1730.14944325907241518No Hit
AGGGCCTGCCTTAAGTGTGAAAGTAATGACTGATGAAAGTGGAAAATCCA1650.14253258813265035No Hit
GCCCTACGCGCAGAGGAGAATGGCGAGTCTGTCTCTTATACACATCTCCG1410.12180057531335574No Hit
GATCCTCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAACT1360.11748140597600269No Hit
ACACATATGGCCTAGACTACGTACATAACCTAAACCTACTCCAATTCTAA1320.11402607050612026No Hit
ACACCCAGCCTTGGCCAGCTGGAACACCTGTCTCTTATACACATCTCCGA1230.10625156569888479No Hit
GATGACTGCGCCGGTGAAGCTTCAGGGGGTTTGGATGAGAATGGCTGTTA1230.10625156569888479No Hit
TGTTGGATCAGGACATCCTGATGGTGCAACCGCTATCAAAGGTTCGTTTG1180.10193239636153174No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)