FastQCFastQC Report
Sun 21 May 2023
EGAF00008003655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115763
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC2551822.043312630114976No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT2044317.659355752701643No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG1665414.386289228855507No Hit
CAACGCGCTCACTTCCTTCGTGGACGCCAGCATGGTGTACGGCAGCGAGG42683.6868429463645556No Hit
GGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT24622.126758981712637No Hit
GTGTTCCAGCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACT13961.2059120789889688No Hit
GTTTGGACCCCAGACCTGCTGAACAACCACATCTTGAAGTCAGCTATGTG11691.0098217910731409No Hit
GGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC6750.5830878605426604No Hit
TGCGGCGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT6190.5347131639643063No Hit
GGCTGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT5070.4379637708075983No Hit
TGCGGTGGGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT4480.3869975726268324No Hit
GTGTTCCAGCTAGCCAAGGCTGGGTGTCTGTCTCTTATACACATCTGACG4470.3861337387593618No Hit
AGCTCCATCTGCATACGACAAACAGACCTAAAATCGCTCATTGCATACTC3850.3325760389761841No Hit
GGCTGTGGGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT3750.323937700301478No Hit
CCGACCTGGCTTCCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG3420.29543118267494795No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGAATGTTGGAGAAATAAAAT3030.2617416618435942No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATAGCATACTC2920.25223948930141754No Hit
TGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC2860.2470564860965939No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGTAATAAAAT2840.2453288183616527No Hit
AGCTCCATCTGCCTACGAGAAACAGACCTAAAATCGCTCATTGCATACTC2790.24100964902429964No Hit
AGCTCCAGCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC2650.22891597487971113No Hit
TGGGTTTTGCCTGCATACGACAAACAGACCTAAAATCGCTCATTGCATAC2490.2150946330001814No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGCAATAAAAT2290.19781795565076923No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATCGCATACTC2280.19695412178329866No Hit
AGCTCCATCTGCCTACGACAAACAAACCTAAAATCGCTCATTGCATACTC2240.1934987863134162No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAATTTAATCG2140.18486044763871012No Hit
CCGACCTGGCTTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG2090.18054127830135708No Hit
CCGACCTGGATTCCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG2030.17535827509653343No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGAGTTTAATCG1920.16585610255435676No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGATTTTAATCG1780.15376242840976823No Hit
GGCTGTGTGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT1770.15289859454229762No Hit
AGCTCCATCTGCCTACGACAAACAGACCGAGAATCGCTCATTGCATACTC1740.1503070929398858No Hit
TGCGGTGTGTGATGCTAGGGTAGAGTCCGAGTATGTTGGAGAAATAAAAT1670.14426025586759156No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGGAATAAAAT1640.14166875426517972No Hit
GTGTTCCAGCTGGACGAGGCTGGGTGTCTGTCTCTTATACACATCTGACG1600.1382134187952973No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGAACACGTAGGACTTTAATCG1580.13648575106035607No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGGGTATGTTGGAGAAATAAAAT1580.13648575106035607No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGGTGGAGAAATAAAAT1460.12611974465070877No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAAATCACGTAGGACTTTAATCG1450.12525591078323817No Hit
AGCTCCATCTGCCTACGACAAACAGTCCTAAAATCGCTCATTGCATACTC1440.12439207691576756No Hit
TGTACCCCCTTCTTCCTGCTGAATACAGACCACTTACTTTCTGTGCATCT1370.11834523984347331No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATGGCATACTC1370.11834523984347331No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAGAATCGCTCATTGCATACTC1360.11748140597600269No Hit
TCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG1330.11488990437359087No Hit
CCGACCTGGATTACTCCGCTCTGAACTCAGATCACGTAGGACTTTAATCG1280.11057073503623782No Hit
CAACGCGCTCACTTCCTTCGTGGACGCCCTCATGGTGTACGGCAGCGAGT1280.11057073503623782No Hit
GGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT1280.11057073503623782No Hit
TGCGGTGTGAGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT1270.10970690116876723No Hit
CAACGCGCTCACTTCCTTCGTGGACGCCCGCATGGTGTACGGCAGCGAGG1260.10884306730129661No Hit
TGCGGTGTGTGATGCTAGCGTAGAATCCGAGTATGTTGGAGAAATAAAAT1230.10625156569888479No Hit
AGCTCCATCTGTCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC1220.10538773183141419No Hit
AGCTCTATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC1210.10452389796394357No Hit
GTGTTCCAGCTGGACAAGGCTGGGTGTCTGTCTCTTATACACATCTGACG1200.10366006409647296No Hit
TGCGGTGTGTGATGCTAGCGTAGAATCCGAGTATGGTGGAGAAATAAAAT1200.10366006409647296No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAACCG1200.10366006409647296No Hit
AGCTCCATCTGCCTACGACAAACAGCCCTAAAATCGCTCATTGCATACTC1190.10279623022900236No Hit
CCGACCTGGATTACTCCGGTCTGCACTCAGATCACGTAGGACTTTAATCG1190.10279623022900236No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGAACACGTAGGACGTTAATCG1170.10106856249406115No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)