FastQCFastQC Report
Sun 21 May 2023
EGAF00008003657

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003657
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39392
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC1151329.226746547522342No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT619715.731620633631193No Hit
CCGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG33558.516957757920391No Hit
GGCTGTGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT21905.559504467912267No Hit
CAACGCGCTCACTTCCTTCGTGGACGCCAGCATGGTGTACGGCAGCGAGG12133.0793054427294884No Hit
GTGTTCCAGCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACT7701.9547116165718927No Hit
TGTACCCCCTTCTTCCTGCTGAATACAGACCACTTACTTTCTGTGCATCT2530.6422623883021933No Hit
TGGGTTTTGCCTGCATACGACAAACAGACCTAAAATCGCTCATTGCATAC2470.6270308692120227No Hit
GTGTTCCAGCTGGACGAGGCCGACGAGGCGGCGGCTGCGGCAGCAGCGGC2000.5077173030056864No Hit
AGCTCCATCTGCATACGACAAACAGACCTAAAATCGCTCATTGCATACTC1700.43155970755483347No Hit
GTTTGGACCCCAGACCTGCTGAACAACCACATCTTGAAGTCAGCTATGTG1400.3554021121039805No Hit
GGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC1220.3097075548334687No Hit
TGCGGTGGGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT1160.29447603574329817No Hit
TGCGGTGTGTGATGCTAGGGTAGAGTCCGAGTATGTTGGAGAAATAAAAT1120.2843216896831844No Hit
AGCTCCAGCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC1090.2767059301380991No Hit
GGCTGTGGGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT1090.2767059301380991No Hit
AGCTCCATCTGCCTACGACAAACAAACCTAAAATCGCTCATTGCATACTC1080.27416734362307066No Hit
GGCTGTGTGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT1080.27416734362307066No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATAGCATACTC1080.27416734362307066No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATCGCATACTC1020.2589358245329001No Hit
GGCTGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT890.22593419983753046No Hit
AGCTCCATCTGCCTACGACAAACAGTCCTAAAATCGCTCATTGCATACTC880.22339561332250205No Hit
AGCTCCATCTGCCTACGAGAAACAGACCTAAAATCGCTCATTGCATACTC870.2208570268074736No Hit
TGCGGCGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT740.18785540211210397No Hit
GGCTGTGGGTGGTTGTGTTGATTAAAATTATGTGTTTTTTGGAAAGTCAT680.17262388302193338No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAGAATCGCTCATTGCATACTC660.1675467099918765No Hit
AGCTCCATCTGCCTACGACAAACAGCCCTAAAATCGCTCATTGCATACTC660.1675467099918765No Hit
TGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC650.1650081234768481No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGTAATAAAAT620.1573923639317628No Hit
TGCGGTGTGTGATGCTAGGGTAGCATCCGAGTATGTTGGAGAAATAAAAT610.15485377741673434No Hit
AGCTCTATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC580.14723801787164909No Hit
TGCGGTGTGTGATGCTAGGGTAGAATCCGAGTATGTTGGAGCAATAAAAT540.13708367181153533No Hit
AGCTCCATCTGCCTACGACAAACAGACCGAGAATCGCTCATTGCATACTC520.13200649878147847No Hit
AGCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATGGCATACTC520.13200649878147847No Hit
CCGACCTGGCTTCCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG510.12946791226645005No Hit
GGCTGCGGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCAT500.1269293257514216No Hit
AGCTCCATCTGCCTACGACAAACTGACCTAAAATCGCTCATTGCATACTC490.12439073923639317No Hit
CAACGCGCTCACTTCCTTCGTGGACGCCCTCATGGTGTACGGCAGCGAGT460.11677497969130787No Hit
TGCGGTGTGAGATGCTAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT450.11423639317627945No Hit
AGCTCCATCTGCCTACGACAAACCGACCTAAAATCGCTCATTGCATACTC450.11423639317627945No Hit
TGCGGTGTGTGATGATAGGGTAGAATCCGAGTATGTTGGAGAAATAAAAT440.11169780666125102No Hit
GGCTGTGGGTGGTTGTGTTGATTCAAATTATGAGTTTTTTGGAAAGTCAT440.11169780666125102No Hit
ATCTCCATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACTC410.10408204711616573No Hit
CGGACCTGGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG400.10154346060113728No Hit
GTTTTCCAGCTGCCTACGACAAACAGACCTAAACTCGCTCATTGCATACT400.10154346060113728No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)