FastQCFastQC Report
Sun 21 May 2023
EGAF00008003801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008003801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34609
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGAAAAGGTGGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT11803.4095177554971245TruSeq Adapter, Index 11 (95% over 21bp)
TCGAAAAGGTGGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT1920.5547689907249559TruSeq Adapter, Index 11 (95% over 21bp)
CCGAAAAGGTGGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATGTCGTATGCCGTCTT1150.3322835100696351No Hit
CCGAAAAGGTGGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGAGCTCGTATGCCGTCTT1130.32650466641625014No Hit
GAGATGTGTATAAGAGACAGGCGATTGGCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGC720.20803837152185847No Hit
CGTCAGATGTGTATAAGAGACAGCCGAAAAGGTGGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCG640.18492299690831865No Hit
GATGTGTATAAGAGACAGGCGATTGGCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG550.15891820046808633No Hit
CCGAAAAGGTGGGAGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATATCGTATGCCGTCTT520.1502499349880089No Hit
TGTGTATAAGAGACAGGCGATTGGCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC430.12424513854777659No Hit
GTGCATGTGGTGGATGCCTTGGCGATTACAGGCGACGAAGGACGTGATAGCCTGCGATAAGCTTCGGGGAGCTGG420.12135571672108411No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)