FastQCFastQC Report
Tue 30 May 2023
EGAF00008004449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008004449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35960
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTGAGGTTGCAGACTGGTCTGAAGGTGTACAGGTGCCCTCTGTGCCTATTCAGCAATTCCCTACTGAAGACT12413.4510567296996664No Hit
AGCCTGAGGTTGCAGACTGGTCTGAAGGTGTGCAGGTGCCCTCTGTGCCTATTCAGCAGTTCCCTACTGAAGACT9802.7252502780867633No Hit
AGCCTGAGGTTGCAGACTGGTCTGAAGGTGTACAGGTGCCCTCTGTGCCTATTCAGCAGTTCCCTACTGAAGACT9352.6001112347052278No Hit
AGCCTGAGGTTGCAGACTGGTCTGAAGGTGTACAGGTGCCCTCTGTGCCTATTCAGTAGTTCCCTACTGAAGACA7001.9466073414905452No Hit
AGCCTGAGGTTGCAGACTGGTCTGAAGGGTGCAGGTATCCTCTGTGCCTATTCAGCAGTTCCCTACTGAAGACTG4471.243047830923248No Hit
AGCCTGAGGTTGCAGACTGGCCTGACGGCGTGCAGGTGCCCTCTGTGCCTATTCAGCAGCTCCCTACTGAAGACT4211.1707452725250278No Hit
AGCCTGAGGTTGCAGACTGGTCTGAAGGTCTGCAGGTGCCCTCTGTGTCTATTCAGTAGTTCCCTACTGAAGACT4201.1679644048943272No Hit
AGCCTGAGGTTGCAGACTGGTCTGAAGGTGTACAGGTGCCCTCTGTGCCTATTCCCTACTGAAGACGGGAGCGCT3711.0317018909899889No Hit
AGCCTGAGGTTGCAGACTGGTCTGACGGCGTTTAGGTGCCCTCTGTGCCTATTCAGAAGTTCCCTACTGAAGACT2240.6229143492769744No Hit
AGAGACTTCCTCCACTGCCAGAGATCTTGAATAGCCTCTTGGTTAGTCATCCGGAAGCAATTCTTCACATAATTG1570.4365962180200222No Hit
AGAGACTTCCTCCACTGCCAGAGATCTTGAATAGCCTCTTGGTCAGTCATCCGGAAGCAATTCTTCACGTAATTG1550.43103448275862066No Hit
AGAGACTTCCTCCACTGCCAGAGATCTTGAACAACCTCCTATTCCCAGTCATCTGGAAGCAATTCTTCACATAAT1500.41713014460511677No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA1180.3281423804226919No Hit
AGCCTGAGGTTGCAGACTGGTCTGACGGCGTGCGGGTGCCCTCTGTGCCTATTCAGCTGTTCCCTACTGAAGACT1100.3058954393770857No Hit
AGAGACTTCCTCCACTGCCAGAGATCTTGAATAGCCTCCTGGTCAGTCATCTGGAAGCAATTCTTCACATAACTG970.26974416017797553No Hit
GCTCCTGGGAAGAGAACCACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC860.23915461624026696No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCT840.23359288097886544No Hit
AGAGACTTCCTCCACTGCCAGAGATCTTGAATAGCCTCTTGGTCAGTCATCCAGAAGAAATTCTTCACATAATTG830.23081201334816462No Hit
AGCCTGAGGTTGCAGACTGGTCTGAAGGTCTGCAGGTACCCTCTGTGTCTATTCAGTAGTTCCCTACTGAAGACT800.22246941045606228No Hit
AGGGTATTTGGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCTGCTTGGCGAGGCAGAGACTGCTGGGC770.21412680756395996No Hit
AGCCTGAGGTTGCAGACTGGTCTGAAGGTGTACAGGTGCCCTCTGTGCCTATTCAGCAATTCCCTACTGGTATGT760.21134593993325915No Hit
AGGGTATTTGGTTTCCCAGTCCACTATACTGACGTCTCCAACATGAGCCGCTTGGCGAGGCAGAGACTGCTGGGC720.20022246941045607No Hit
AGCCTGAGGTTGCAGACTGGTCTGCAGCTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGA710.1974416017797553No Hit
AGCCTGAGGTTGCAGACTGGTCTGAAGGTGTACAGGTGCCCTCTGTGCCTATTCAGCAGTTCCCTACTGACGACT680.18909899888765294No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCA640.17797552836484984No Hit
GCTCCTGGGAACTCGAGAACACCGAGATGAGGCCCGGGGCCGACGCGGCCATGGCGAGGGCGGCGGCGGGGAGGA600.16685205784204674No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGA540.15016685205784205No Hit
AGAGACTTCCTCCACTGCCAGAGATCTTGAATAGCCTCTTGGTCAGTCATCCAGAAGCAATTCTTCACAAAATTG480.13348164627363737No Hit
AGCCTGAGGTTGCAGCTCCCCTGTCTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG450.12513904338153503No Hit
GCTCCTGGGAACTCGAGAACACCGAGATGAGGCCCGGGGCCGGCGCGGCCATGGCGAGGGCGGCGGCGGGGAGGA400.11123470522803114No Hit
GTATCAACGCAGAGTACATGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGC400.11123470522803114No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACAT390.10845383759733036No Hit
GGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT390.10845383759733036No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGG390.10845383759733036No Hit
GAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAAT380.10567296996662957No Hit
AGAGACTTCCTCCACTGCCAGAGATCTTGAATAGCCTCCTGGTCAGTTATCCAGAAGCCATTATTCACGCAATTG370.10289210233592881No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)