FastQCFastQC Report
Sun 21 May 2023
EGAF00008004830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008004830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2684
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTGAGGTTGCAGACTGGTCTGAAGGTGTACAGGTGCCCTCTGTGCCTATTCAGCAATTCCCTACTGAAGACT220.819672131147541No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGG130.48435171385991055RNA PCR Primer, Index 27 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG80.29806259314456035RNA PCR Primer, Index 27 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGG70.26080476900149036RNA PCR Primer, Index 27 (96% over 28bp)
GTATAAGAGACAGCGTAGGGAGCGCTTCCTACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGACCCT60.22354694485842028No Hit
TGCCTGAGGTTGCAGACTGGTCTGAAGGTGTACAGGTGCCCTCTGTGCCTATTCAGCAATTCCCTACTGAAGACT50.18628912071535023No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGG50.18628912071535023No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCTCTG50.18628912071535023No Hit
GGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGA50.18628912071535023No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGG50.18628912071535023RNA PCR Primer, Index 27 (96% over 28bp)
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTC40.14903129657228018No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGC40.14903129657228018No Hit
GAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGG40.14903129657228018No Hit
AAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT40.14903129657228018No Hit
GAGTACATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGT40.14903129657228018No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG40.14903129657228018No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATG40.14903129657228018No Hit
GTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGCC40.14903129657228018No Hit
AATGAAAACATTCTTGGCAAATGCTTTCGCTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCG40.14903129657228018No Hit
GATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGCTGC40.14903129657228018No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTATTGT40.14903129657228018No Hit
GGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGA30.11177347242921014No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGG30.11177347242921014RNA PCR Primer, Index 27 (96% over 28bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA30.11177347242921014No Hit
GGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAG30.11177347242921014No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA30.11177347242921014No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGG30.11177347242921014RNA PCR Primer, Index 27 (96% over 28bp)
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGC30.11177347242921014No Hit
GTATAAGAGACAGCGTAGGGAGCGCTTCCTACGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGACCCG30.11177347242921014No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGT30.11177347242921014No Hit
GAATGTTTTCATTAATCAAGAACGAAAGTCGGAGGTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAA30.11177347242921014No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAAC30.11177347242921014No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTTTTAATGCAGGTTTGG30.11177347242921014No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGT30.11177347242921014No Hit
GGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAA30.11177347242921014No Hit
CTGTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGC30.11177347242921014No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCA30.11177347242921014No Hit
GCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATT30.11177347242921014No Hit
ACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGG30.11177347242921014RNA PCR Primer, Index 27 (96% over 28bp)
CTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGC30.11177347242921014No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGC30.11177347242921014No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTA30.11177347242921014No Hit
GTGTATAAGAGACAGGGCATTGCCCCTGACTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTA30.11177347242921014No Hit
ATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTCA30.11177347242921014No Hit
GTATAAGAGACAGGGCATTGCCCCTGACTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATG30.11177347242921014No Hit
GCTCCTGGGAAGAGAACCACCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCC30.11177347242921014No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGA30.11177347242921014No Hit
ATACACATCTCCGAGCCCACGAGACTTGACCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATGGGGGGG30.11177347242921014RNA PCR Primer, Index 27 (96% over 28bp)
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGT30.11177347242921014No Hit
ATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCA30.11177347242921014No Hit
CCTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGG30.11177347242921014No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)